MABC186-I
Anti-phospho-p62 (Ser403) Antibody, clone 4F6
clone 4F6, from rat
Sinônimo(s):
Ubiquitin-binding protein p62, p62, EBI3-associated protein of 60 kDa, EBIAP, p60, Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.43
Produtos recomendados
fonte biológica
rat
Nível de qualidade
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
4F6, monoclonal
reatividade de espécies
human, mouse
técnica(s)
immunohistochemistry: suitable
western blot: suitable
Isotipo
IgG2aκ
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
ambient
modificação pós-traducional do alvo
phosphorylation (pSer403)
Informações sobre genes
human ... SQSTM1(8878)
Descrição geral
Sequestosome-1 (UniProt: Q13501; also known as EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62) is encoded by the SQSTM1 (also known as ORCA, OSIL) gene (Gene ID 8878) in human Sequestosome is a multi-functional co-interacting protein with a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chains and regulates the activation of the nuclear factor kappa-B (NF-kappaB) signaling. It functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 (TRAF6) to mediate the activation of NF- B in response to upstream signals. It also serves as an autophagosome cargo protein that targets other proteins that bind to it for selective autophagy. Defects in sequestosome-1 are a cause of Paget disease of bone (PDB), a metabolic bone disease affecting the axial skeleton and is characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Mutations in SQSTM1 gene can lead to frontotemporal dementia and/or amyotrophic lateral sclerosis 3. Frontotemporal dementia is characterized by frontal and temporal lobe atrophy associated with neuronal loss, gliosis, and dementia. Amyotrophic lateral sclerosis is characterized by the death of motor neurons in the brain, brainstem, and spinal cord, resulting in fatal paralysis.
Especificidade
Clone 4F6 recognizes p62/SQSTM1 phosphorylated at Ser403 in human and mouse.
Imunogênio
KLH-conjugated linear peptide corresponding to a sequence surrounding pSer403 of human phospho p62/SQSTM1.
Aplicação
Detect p62/SQSTM1 (Ser403) using this rat monoclonal Anti-phospho-p62 (Ser403) antibody, clone 4F6, Cat. No. MABC186-I. Validated for use in Immunohistochemistry and Western Blotting.
Western Blotting Analysis: A representative lot detected p62/SQSTM1 (Ser403) in a Western Blot application. (Matsumoto, G., et. al. (2011). Mol Cell. 44(2):279-289).
Immunohistochemistry Analysis: A representative lot detected p62/SQSTM1 (Ser403) in a Immunohistochemistry application. (Matsumoto, G., et. al. (2011). Mol Cell. 44(2):279-289).
Immunohistochemistry Analysis: A representative lot detected p62/SQSTM1 (Ser403) in a Immunohistochemistry application. (Matsumoto, G., et. al. (2011). Mol Cell. 44(2):279-289).
Qualidade
Evaluated by Western Blotting in Neuro2a cells.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected p62/SQSTM1 (Ser403) in Neuro2a cells in which GFP fused human p62 (G-p62) was stably transfected and treated with MG132 vs. an untreated sample.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected p62/SQSTM1 (Ser403) in Neuro2a cells in which GFP fused human p62 (G-p62) was stably transfected and treated with MG132 vs. an untreated sample.
Descrição-alvo
~60 kDa observed; 46.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
forma física
Format: Purified
Outras notas
Concentration: Please refer to lot specific datasheet.
Não está encontrando o produto certo?
Experimente o nosso Ferramenta de seleção de produtos.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Valeria Gerbino et al.
Neuron, 106(5), 789-805 (2020-03-30)
DNA sequence variants in the TBK1 gene associate with or cause sporadic or familial amyotrophic lateral sclerosis (ALS). Here we show that mice bearing human ALS-associated TBK1 missense loss-of-function mutations, or mice in which the Tbk1 gene is selectively deleted
Zhiqiang Deng et al.
Autophagy, 16(5), 917-931 (2019-08-01)
Macroautophagy (autophagy) is a key catabolic pathway for the maintenance of proteostasis through constant digestion of selective cargoes. The selectivity of autophagy is mediated by autophagy receptors that recognize and recruit cargoes to autophagosomes. SQSTM1/p62 is a prototype autophagy receptor
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica