MABC182

Anti-NRF-1 Antibody, clone 10B11.1

clone 10B11.1, from mouse

• Sinônimo(s): Nuclear respiratory factor 1, Alpha-pal, Alpha palindromic-binding protein, NRF-1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 10B11.1, monoclonal
• reatividade de espécies: mouse
• reatividade da espécie (prevista por homologia): human (based on 100% sequence homology)
• técnica(s): western blot: suitable
• Isotipo: IgG2bκ
• nº de adesão NCBI: NP_001035199
• nº de adesão UniProt: Q16656
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... NRF1(4899)

Descrição

Descrição geral

Nuclear respiratory factor 1 (UniProt Q16656; also known as Alpha-pal, Alpha palindromic-binding protein, NRF-1) is encoded by the NRF1 (Also known as ALPHA-PAL) gene (Gene ID 4899) in human. NRF-1 is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. NRF-1 is upregulated and activated/phosphorylated by Akt in response to oxidant exposure or increased cellular reactive oxygen species (ROS), resulting in NRF-1 nuclear translocation, where it binds to DNA as a homodimer and mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.

Especificidade

Immunogen construct region is present in human NRF-1 spliced isoform Long, isfoform Short, and isfoform 4, but is more than 40% absent from spliced isoform 4 reported by UniProt (Q16656).

Imunogênio

Epitope: Internal (N-terminal half).

GST-tagged recombinant human NRF-1 internal fragment.

Aplicação

Research Category
Apoptosis & Cancer

Research Sub Category
Transcription Factors

This Anti-NRF-1 Antibody, clone 10B11.1 is validated for use in Western Blotting for the detection of NRF-1.

Qualidade

Evaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected NRF-1 in 10 µg of NIH/3T3 cell lysate.

Descrição-alvo

~58 kDa observed. 53.54/53.57 kDa (human/mouse isoform Long), 46.16 kDa (human/mouse isoform Short), 36.05 kDa (human isoform 3), 55.67 kDa (human isoform 4) calculated.

forma física

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable