MABC1227

Anti-PIP4K2B (PIP5K2B) Antibody, clone 7D10.1

clone 7D10.1, from mouse

• Sinônimo(s): Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, 1-phosphatidylinositol 5-phosphate 4-kinase 2-beta, Diphosphoinositide kinase 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, PI(5)P 4-kinase type II beta, PIP4KII-beta, PtdIns(5)P-4-
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propriedades

• fonte biológica: mouse
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 7D10.1, monoclonal
• reatividade de espécies: mouse
• reatividade da espécie (prevista por homologia): human (based on 100% sequence homology)
• técnica(s): western blot: suitable
• Isotipo: IgG2aκ
• nº de adesão NCBI: NP_003550
• nº de adesão UniProt: P78356
• Condições de expedição: ambient
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PIP4K2B(8396)

Descrição

Descrição geral

Phosphatidylinositol 5-phosphate 4-kinase type-2 beta (EC 2.7.1.149; UniProt P78356; also known as 1-phosphatidylinositol 5-phosphate 4-kinase 2-beta, Diphosphoinositide kinase 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, PI(5)P 4-kinase type II beta, PIP4KII-beta, PIP4K2B, PtdIns(5)P-4-kinase isoform 2-beta) is encoded by the PIP4K2B (also known as PIP5K2B, PIP5KIIB) gene (Gene ID 8396) in human. PIP4K2B catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 5-phosphate to 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate. It is highly expressed in brain, heart, pancreas, skeletal muscle and kidney, however, lower levels are detected in placenta, lung, and liver. Mice deficient in PIP4K2B are shown to be hypersensitive to insulin and exhibit reduced body weights compared to wild-types. Adult male mice lacking PIP4K2B have significantly less body fat, but female mice show increased sensitivity to insulin in the presence of normal adiposity. Mice lacking PIP4K2B exhibit enhanced in vivo insulin-induced activation of Akt in the skeletal muscle and liver. Ref.: Lamia, KA., et al. (2004). Mol. Cell 24, 5080-5087

Especificidade

Epitope region is present in spliced isoform 1, but not in isoform 2 reported by UniProt (P78356).

Imunogênio

GST-tagged recombinant fragment corresponding to a region within the C-terminal half of human PIP4K2B (PIP5K2B).

Aplicação

Detect PIP4K2B using this mouse monoclonal Anti-PIP4K2B (PIP5K2B) Antibody, clone 7D10.1, Cat. No. MABC1227, validated for use in Western Blotting.

Research Category
Apoptosis & Cancer

Qualidade

Evaluated by Western Blotting in L-929 mouse fibroblast lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PIP4K2B (PIP5K2B) in 10 µg of L-929 mouse fibroblast lysate.

Descrição-alvo

~48 kDa observed. 47.25/47.19 kDa (human/mouse) calculated (Met 1 removed). Uncharacterized bands may be observed in some lysate(s).

forma física

Format: Purified

Protein G purified.

Purified mouse IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable