MAB3408
Anti-Tubulin Antibody, beta, clone KMX-1
clone KMX-1, Chemicon®, from mouse
Sinônimo(s):
Tubulin beta-III, tubulin, beta 3, tubulin, beta 4
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
KMX-1, monoclonal
reatividade de espécies
human, rat, mouse
reatividade da espécie (prevista por homologia)
all
fabricante/nome comercial
Chemicon®
técnica(s)
immunocytochemistry: suitable
western blot: suitable
Isotipo
IgG2b
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... TUBB3(10381)
Descrição geral
Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues.
Especificidade
This antibody reacts specifically with β-tubulin and shows a strong cross-reactivity to different species. This antibody allows staining of microtubuli using indirect immunofluorescence showing a low background only.
Imunogênio
Tubulin purified from polycephalum myxamoebae
Aplicação
Immunocytochemistry:
A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry.
Western blot:
0.05-0.5 μg/mL
Optimal working dilutions must be determined by end user.
A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry.
Western blot:
0.05-0.5 μg/mL
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-Tubulin Antibody, beta, clone KMX-1 is validated for use in IC, WB for the detection of Tubulin.
Qualidade
Routinely evaluated by Western Blot on A431 lysates.
Western blot:
1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates.
Western blot:
1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates.
Descrição-alvo
50 kDa
Ligação
Replaces: 04-1049
forma física
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2b liquid in buffer containing 0.02M phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Armazenamento e estabilidade
Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
Nota de análise
Control
HeLa Cell lysate
A431 Cell lysate
MCF7 Cell lysate
HeLa Cell lysate
A431 Cell lysate
MCF7 Cell lysate
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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