MAB10234-I

Anti-Hepatitis B Virus Surface Antigen Antibody, clone H21F8-1

• Código UNSPSC: 12352203
• NACRES: NA.74

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• conjugado: unconjugated
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: H21F8-1, monoclonal
• peso molecular: calculated mol wt 43.72 kDa; observed mol wt ~43.72 kDa
• reatividade de espécies: virus
• embalagem: antibody small pack of 100 μg
• técnica(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• Isotipo: IgM
• sequência de epítopo: Unknown
• nº de adesão UniProt: A7XR30
• Condições de expedição: 2-8°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: vaccinia virus ... S(944569)

Descrição

Descrição geral

Large envelope protein (UniProt: A7XR30; also known as L glycoprotein, L-HBsAg, LHB, Large S protein, Large surface protein, Major surface antigen) is encoded by the S (also known as Large S, P, preS1, preS2) gene (Gene ID: 944569) in Hepatitis B virus (HBV). HBV is a small enveloped virus of the Hepadnaviridae family with a partially double-stranded DNA genome approximately 3.2 kb. Hepatitis B surface antigen is a distinctive serological marker of acute or chronic hepatitis B infection and is the first antigen to appear following infection with hepatitis B virus. HBV contains three viral envelope/surface proteins - large surface antigen, middle surface antigen, and small surface antigen. They are expressed from a single open reading frame. The large envelope protein is synthesized with the pre-S region at the cytosolic side of the endoplasmic reticulum and is not N-glycosylated. It is shown to exist in two topological conformations, one which is termed ′external′ or Le-HBsAg and the other ′internal′ or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein. (Ref.: Wu, CC., et al. (2018). World J. Gastroenterol. 24(31);: 3488 3499).

Especificidade

Clone H21F8-1 is a mouse monoclonal antibody that detects large envelope protein of Hepatitis B virus.

Imunogênio

Hepatitis B virus surface antigen (HBsAg).

Aplicação

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in Hepatitis B virus infected liver (HBsAg-positive) tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Hepatitis B Virus Surface Antigen in Hepatitis B virus infected liver (HBsAg-positive) tissue sections.

Anti-Hepatitis B Virus Surface Antigen, clone H21F8-1, Cat. No. MAB10234-I, is a mouse monoclonal antibody that detects Large envelope protein of Hepatitis B virus and is tested for use in Immunohistochemistry (Paraffin).

forma física

Purified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable