IP02

Protein A Agarose Suspension

Name: Protein A Agarose Suspension
Brand: MM

Protein A Agarose Suspension designed for immunoprecipitation applications

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Sobre este item

NACRES:

NA.56

UNSPSC Code:

41116133

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form

slurry (Liquid)

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem^®

storage condition

do not freeze

shipped in

wet ice

storage temp.

2-8°C

Categorias relacionadas

Antibody Protein Purification

Protein Purification Chromatography

Protein Sample Prep

Protein Biology

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Este Item	IP05	IP04	P1925
Millipore IP02 Protein A Agarose Suspension	Millipore IP05 Protein G Plus/Protein A Agarose Suspension	Millipore IP04 Protein G Plus-Agarose Suspension	Millipore P1925 Protein A–Agarose macrobeads
form slurry (Liquid)	form slurry (Liquid)	form slurry (Liquid)	form aqueous suspension
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
contains ≤0.1% sodium azide as preservative	contains ≤0.1% sodium azide as preservative	contains ≤0.1% sodium azide as preservative	contains -
manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename -
storage condition do not freeze	storage condition do not freeze	storage condition do not freeze	storage condition -
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in -

General description

Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for mouse IgG_2a and IgG_2b and rabbit IgG.

Protein A conjugated to agarose.

Physical form

In PBS.

Other Notes

Agarose solutions are supplied ready-to-use for immunoprecipitation. Use 15 µl suspension/µg primary antibody. Protein A-Agarose is recommended for use in immunoprecipitation assays using rabbit polyclonal antibodies or unmodified mouse IgG_2a or IgG_2b monoclonal antibodies. Mouse IgG₁ or IgG₃ and all rat antibodies bind poorly to Protein A. As an alternative, use Protein G PLUS, Protein G PLUS/Protein A, or rabbit second-step antibodies to bridge these antibodies to Protein A. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Classe de armazenamento

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protein arginine methyltransferase expression, localization, and activity during disuse-induced skeletal muscle plasticity.

Derek W Stouth et al.

American journal of physiology. Cell physiology, 314(2), C177-C190 (2017-11-03)

Protein arginine methyltransferase 1 (PRMT1), PRMT4, and PRMT5 catalyze the methylation of arginine residues on target proteins. Previous work suggests that these enzymes regulate skeletal muscle plasticity. However, the function of PRMTs during disuse-induced muscle remodeling is unknown. The purpose

p38β Mitogen-Activated Protein Kinase Modulates Its Own Basal Activity by Autophosphorylation of the Activating Residue Thr180 and the Inhibitory Residues Thr241 and Ser261.

Jonah Beenstock et al.

Molecular and cellular biology, 36(10), 1540-1554 (2016-03-16)

Many enzymes are self-regulated and can either inhibit or enhance their own catalytic activity. Enzymes that do both are extremely rare. Many protein kinases autoactivate by autophosphorylating specific sites at their activation loop and are inactivated by phosphatases. Although mitogen-activated

GemC1 governs multiciliogenesis through direct interaction with and transcriptional regulation of p73.

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Journal of cell science, 132(11) (2019-04-28)

A distinct combination of transcription factors elicits the acquisition of a specific fate and the initiation of a differentiation program. Multiciliated cells (MCCs) are a specialized type of epithelial cells that possess dozens of motile cilia on their apical surface.

Differential binding modes of the bromodomains of CREB-binding protein (CBP) and p300 with acetylated MyoD.

Lanlan Wei et al.

Biochemical and biophysical research communications, 368(2), 279-284 (2008-01-29)

The recruitment of the bromodomains of CREB-binding protein (CBP) and p300 by the acetylated myogenic transcription factor MyoD was previously shown to be critical for the enhanced MyoD transcriptional activity following acetylation at its Lys99 and Lys102 positions. However, the

Registered report: RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth.

Ajay Bhargava et al.

eLife, 5 (2016-02-18)

The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010

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Agarose Beads for Immunoprecipitation and Antibody Purification

We manufacture IP and antibody purification agarose beads that purify immunoglobulins and IgG fractions. Our kits also provide a total antibody purification process

Número de item de comércio global

SKU	GTIN
IP02-1.5ML	04055977220209

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