IM42

Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6)

liquid, clone 114-6G6, Calbiochem^®

• Sinônimo(s): Anti-MMP-14
• Código UNSPSC: 12352203

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 114-6G6, monoclonal
• Formulário: liquid
• contém: ≤0.1% sodium azide as preservative
• reatividade de espécies: human
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; avoid repeated freeze/thaw cycles
• Isotipo: IgG1
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... MMP14(4323)

Descrição

Descrição geral

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~66 kDa latent and the ~60 kDa active forms of MT1-MMP protein.

Recognizes the ~60-66 kDa MTI-MMP protein in HT-1080 cells and ConA-treated MD-MB-231 cells.

This Anti-MT1-MMP (Ab-3) Mouse mAb (114-6G6) is validated for use in Frozen Sections, Paraffin Sections, Immunoblotting, Immunocytochemistry, IP for the detection of MT1-MMP (Ab-3).

Imunogênio

a synthetic peptide (REVPYAYIREGHEK) corresponding to amino acids 160-173 in the catalytic domain of human MT1-MMP

Aplicação

Frozen Sections (not recommended)
Paraffin Sections (10 g/ml, Pronase<TMSYMBOL></TMSYMBOL> or heat pre-treatment required)
Immunoblotting (10 g/ml)
Immunocytochemistry (see application reference)
Immunoprecipitation (not recommended)

Embalagem

Please refer to vial label for lot-specific concentration.

Advertência

Toxicity: Standard Handling (A)

forma física

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Reconstituição

Following initial thaw, aliquot and freeze (-20°C).

Nota de análise

Negative Control
trpE (Ab-1) or untreated MD-MB-231 cells

Positive Control
HT-1080 cells, ConA-treated MD-MB-231 cells, or breast carcinoma tissue

Outras notas

Does not cross-react with MT2-MMP or MT3-MMP. To induce MT1-MMP in MD-MB-231 cells, treat with 1-5 µg/ml ConA for 24-48 h. For best results with paraffin sections, fix with perioddate-lysine-4% paraformaldehyde. Antibody should be titrated for optimal results in individual systems.

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Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Sem. Cancer Biol., ed. M.M. Gottesman. Vol. 1(2):99-106.
Goldberg, G.I., et al. 1989. PNAS86, 8207.

Informações legais

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1