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Key Documents

HTS171RTA

Sigma-Aldrich

Ready-to-Assay sst 3 Somatostatin Family Receptor Frozen Cells

Human

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About This Item

Código UNSPSC:
41106514
eCl@ss:
32011203
NACRES:
NA.81

product name

Ready-to-Assay sst 3 Somatostatin Family Receptor Frozen Cells,

fonte biológica

human

Nível de qualidade

fabricante/nome comercial

Ready-to-Assay

técnica(s)

calcium flux assay: suitable

nº de adesão NCBI

método de detecção

fluorometric

Descrição geral

Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Somatostatin is a 14 or 28 amino acid regulatory peptide that inhibits hormone secretion from the pituitary, pancreas, and other endocrine sites. A family of 6 GPCRs, sst1, sst2A, sst2B, sst3, sst4 and sst5, mediate the biological activity of somatostatins. The somatostatin receptors couple to Gi to inhibit cAMP production, and also increase MAP kinase signalling. Several tumors have been shown to overexpress somatostatin receptors, and binding of somatostatin to these tumor cells stimulates or inhibits proliferation, depending on the receptor subtypes expressed (Olias et al., 2004). However, sst3 appears to promote apoptosis, and expression of sst3 was found to be lower in gastric cancer cells than in normal gastric mucosa, in proportion to susceptibility to apoptosis induced by somatostatin analogs (Sharma et al., 1996; Hu et al., 2004). In addition, sst3 is expressed in smooth muscle cells and mediates gastrointestinal contractions. Millipore′s cloned human sst3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant sst3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at sst3.

Descrição de linhagem celular

  • GPCR Cell Lines
  • Host cells: Chem-1

Aplicação

Human sst3 GPCR frozen cells for Calcium Flux FLIPR Assays.

Ações bioquímicas/fisiológicas

  • GPCR Class: A
  • Protein Target: sst3
  • Target Sub-Family: Somatostatin

Componentes

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.

Exoneração de responsabilidade


This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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