HTS096M
ChemiSCREEN Human α2A Adrenergic Receptor Membrane Preparation
Human alpha2A GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
Sinônimo(s):
High GPCR membrane prep
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About This Item
Código UNSPSC:
41106514
eCl@ss:
32161000
NACRES:
NA.84
Produtos recomendados
fonte biológica
human
Nível de qualidade
recombinante
expressed in Chem-1 cells
fabricante/nome comercial
ChemiScreen
Chemicon®
técnica(s)
ligand binding assay: suitable (GTPγS)
radioligand binding assay (RLBA): suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
Informações sobre genes
human ... ADRA2A(150)
Descrição geral
Full-length human ADRA2A transcript variant 1 cDNA encoding α2A
The endogenous catecholamines epinephrine and norepinephrine have profound effects on smooth muscle activity, cardiac function, carbohydrate and fat metabolism, hormone secretion, neurotransmitter release, and central nervous system actions. These activities are mediated by GPCRs belonging to two subfamilies, the α- and β-adrenoceptors (Bylund et al., 1994). The α2 adrenergic receptor subfamily members, consisting of α2A, α2B, and α2C, couple primarily to Gi to inhibit cAMP production, and play an important role in regulation of cardiovascular and CNS function. Experiments with α2A-selective agonists and mice lacking α2A demonstrate that activation of α2A results in hypotension, sedation, analgesia, and hypothermia (Kable et al., 2000). Chemicon′s α2A membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at α2A. The membrane preparations exhibit a Kd of 5.4 nM for [3H]-MK-912. With 5 nM [3H]-MK-912, 5 µg/well α2A Membrane Prep typically yields greater than 20-fold signal-to-background ratio.
Aplicação
Human alpha2A GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
Radioligand binding assay and GTPγS binding.
Ações bioquímicas/fisiológicas
GPCR Class: A
Protein Target: alpha2A
Target Sub-Family: Adrenergic
Qualidade
Table 1. Signal:background and specific binding values obtained in a competition binding assay with α2A membrane prep and unlabeled rauwolscine.
SPECIFICATIONS: 1 unit = 5 µg
Bmax for [3H] MK-912 binding: 71.4 pmol/mg protein
Kd for [3H] MK-912 binding: ~5.4 nM
| 10 µg/well | 5 µg/well | |
|---|---|---|
| Signal:Background | 28.97 | 34.14 |
| Specific Binding (cpm) | 11777 | 9147 |
SPECIFICATIONS: 1 unit = 5 µg
Bmax for [3H] MK-912 binding: 71.4 pmol/mg protein
Kd for [3H] MK-912 binding: ~5.4 nM
Especificações
Inucbation Conditions
Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [3H] MK-912 (Perkin Elmer#: NET-1059)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [3H] MK-912 (Perkin Elmer#: NET-1059)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
forma física
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 20-fold signal:background with 3H-labeled MK-912 at 5 nM
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Armazenamento e estabilidade
Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
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J W Kable et al.
The Journal of pharmacology and experimental therapeutics, 293(1), 1-7 (2000-03-29)
Mice with altered alpha(2)-adrenergic receptor genes have become important tools in elucidating the subtype-specific functions of the three alpha(2)-adrenergic receptor subtypes because of the lack of sufficiently subtype-selective pharmacological agents. Mice with a deletion (knockout) of the alpha(2A)-, alpha(2B)-, or
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