HTS059M

ChemiScreen V1A Vasopressin Membrane Preparation

• Código UNSPSC: 41106514
• eCl@ss: 32161000
• NACRES: NA.45

Propriedades

• fonte biológica: human
• Nível de qualidade: 100
• recombinante: expressed in Chem-1 cells
• forma: liquid
• fabricante/nome comercial: ChemiScreen; Chemicon^®
• técnica(s): ligand binding assay: suitable (GTPγS); radioligand binding assay (RLBA): suitable
• nº de adesão NCBI: NM_000706.3
• nº de adesão UniProt: P37288

Descrição

Descrição geral

Arginine vasopressin (AVP) is a 9 amino acid peptide that functions as an antidiuretic, vasoconstrictor and neurotransmitter. The three vasopressin receptors, V_1A, V_1B and V₂, are GPCRs; V_1A and V_1B couple to G_q and calcium release, whereas V₂ couples to G_s. V₁ receptors regulate fluid homeostasis by stimulating contraction of glomerular mesangial cells and renal vascular smooth muscle cells (Birnbaumer, 2000). Chemicon′s V_1A membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of V_1A interactions with its ligands. The membrane preparations exhibit a Kd of 55 pM for [¹²⁵I]-vasopressin antagonist ([¹²⁵I]-phenylacetyl-D-Tyr(Me)-Phe-Gln-Asn-Arg -Pro-Arg-Tyr-NH2). With 10 μg/well V_1A Membrane Prep and 0.1 nM [¹²⁵]-vasopressin V_1A antagonist, greater than 12-fold signal-to-background ratio were obtained.

Human AVPR1 cDNA encoding V_1A

Aplicação

Human V1A GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

Radioligand binding assay, and GTPγS binding.

Ações bioquímicas/fisiológicas

• GPCR Class: A
• Protein Target: V1A
• Target Sub-Family: Vasopressin

Embalagem

1 mL, 2 mg/mL

forma física

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives. Packaging method: Membranes protein was adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.

Armazenamento e estabilidade

Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2