HTS047M
ChemiScreen A1 Membrane Preparation
Human A1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
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About This Item
Código UNSPSC:
41106514
eCl@ss:
32161000
NACRES:
NA.84
fonte biológica
human
recombinante
expressed in Chem-3 cells
embalagem
pkg of 1 mL
fabricante/nome comercial
ChemiScreen
Chemicon®
concentração
0.5 mg/mL
técnica(s)
ligand binding assay: suitable (GTPγS)
radioligand binding assay (RLBA): suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
Descrição geral
Extracellular adenosine mediates a multitude of biological effects, including wakefulness, antiarrythmia, bronchoconstriction and response to ischemia and oxidative stress. A family of four GPCR adenosine receptors, A1, A2A, A2B and A3, is responsible for these effects. The A1 receptor, which couples to Gi/o, is most highly expressed in brain, and mediates endogenous antinociception and neuronal response to hypoxia (Fredholm et al., 2001). A1 is also expressed in kidney, where it contributes to tubuloglomerular feedback. Chemicon′s cloned human A1-expressing cell line is made in the Chem-3 host, which supports high levels of recombinant A1 expression on the cell surface and contains high levels of the high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between A1 and its ligands.
Chemicon′s A1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of A1 interactions with its ligands. The membrane preparations exhibit a Kd of 1.2 nM for [3H]-Cyclopentyl-1, 3-dipropylxanthine, 8-[dipropyl-2, 3-3H (N)]. With 5mg/well A1 Membrane Prep and 20nM [3H]-DPCPX, a greater than 6-fold signal-to-background ratio is obtained.
Chemicon′s A1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of A1 interactions with its ligands. The membrane preparations exhibit a Kd of 1.2 nM for [3H]-Cyclopentyl-1, 3-dipropylxanthine, 8-[dipropyl-2, 3-3H (N)]. With 5mg/well A1 Membrane Prep and 20nM [3H]-DPCPX, a greater than 6-fold signal-to-background ratio is obtained.
Ações bioquímicas/fisiológicas
Protein Target: A1
Target Sub-Family: Adenosine
Armazenamento e estabilidade
Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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