ABT282

Anti-phospho-NUMA1 Antibody (Thr2055)

from rabbit, purified by affinity chromatography

• Sinônimo(s): Nuclear mitotic apparatus protein 1, NuMA protein, SP-H antigen
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human
• reatividade da espécie (prevista por homologia): mouse (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
• técnica(s): inhibition assay: suitable (peptide); western blot: suitable
• nº de adesão NCBI: NP_006176
• nº de adesão UniProt: Q14980
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: phosphorylation (pThr2055)
• Informações sobre genes: human ... NUMA1(4926)

Descrição

Descrição geral

NUMA1 or NUMA protein is an interesting and major component of the nuclear matrix of eukaryotic cells. NUMA1 is a highly abundant protein component that appears to serve a non-structural role but is needed for mitotic spindle poles and centrosome formation as well as a linker of microtubules. These functions are important for proper cytokinesis and the setup of the proper polarity axis during cell division. NUMA1 resides in the nuclear matrix during interphase. NUMA1 dissociates from condensing chromosomes during early prophase, and relocates to the spindle poles via a dynein/dynamin complex, and it remains there until the anaphase onset. Thus NUMA1 then can be found in the nucleus during cell rest and cytoplasmically and centrosome localized during cytokinesis. NUMA1 phosphorylation occurs by CDK1 and phosphorylation increases solubility and promotes association with dynein and subsequent translocation to the spindle poles.

Imunogênio

Epitope: Tail Region

KLH-conjugated linear peptide corresponding to the tail region of human NUMA1 phosphorylated at Thr2055.

Aplicação

Peptide Inhibition Analysis: A 1:500 dilution from a representative lot blocked NUMA1 phosphorylated at Thr2055 in Hydroxyurea treated HeLa nuclear extract.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-phospho-NUMA1 Antibody (Thr2055) is validated for use in Western Blotting and Peptide Inhibition Assay for the detection of phospho-NUMA1 (Thr2055).

Qualidade

Evaluated by Western Blotting in HeLa nuclear extract and Hydroxyurea treated HeLa nuclear extract.

Western Blotting Analysis: A 1:500 dilution of this antibody detected NUMA1 phosphorylated at Thr2055 in 10 µg of HeLa nuclear extract and Hydroxyurea treated HeLa nuclear extract.

Descrição-alvo

~230 kDa observed

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable