ABE598

Anti-TCF12 Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): HTF4, HEB, BHLHB20, Transcription factor 12, TCF-12, Class B basic helix-loop-helix protein 20, bHLHb20, DNA-binding protein HTF4, E-box-binding protein, Transcription factor HTF-4
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human
• técnica(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• nº de adesão UniProt: Q99081
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... TCF12(6938)

Descrição

Descrição geral

TCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family. TCF12 is a transcriptional regulator involved in the initiation of neuronal differentiation, and it has been shown to activate transcription by binding to the E box (5′-CANNTG-3′). TCF12 has been shown to interact with PTF1A, NEUROD2 and RUNX1T1. TCF12 is suggested to play a critical role in proper T cell differentiation by blocking the thymocyte proliferation in combination with E2A. Recent studies have shown that TCF12 is also an important factor required for the development of invariant natural killer T cells. TCF12 is expressed in many tissues, and more specifically in skeletal muscle, thymus, B- and T-cells.

Imunogênio

KLH-conjugated linear peptide corresponding to human TCF12.

Aplicação

Detect TCF12 using this rabbit polyclonal antibody, Anti-TCF12 Antibody validated for use in western blotting, ICC & IHC.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected TCF12 in human tonsil tissue.

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected TCF12 in HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Qualidade

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TCF12 in 15 µg of HeLa cell lysate.

Descrição-alvo

~83 kDa observed. Uncharacterized bands may appear in some lysates. Uniprot describes 3 isoforms between ~55 kDa and ~76 kDa

forma física

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
HeLa cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable