ABE491

Anti-Histone-lysine N-methyltransferase NSD3 Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): Histone-lysine N-methyltransferase NSD3, Nuclear SET domain-containing protein 3, Protein whistle, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine, Wolf-Hirschhorn syndrome candidate 1-like protein 1, WHSC1-like protein 1
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human, mouse
• técnica(s): immunohistochemistry: suitable; western blot: suitable
• nº de adesão NCBI: NP_075447
• nº de adesão UniProt: Q9BZ95
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... WHSC1L1(54904)

Descrição

Descrição geral

Histone-lysine N-methyltransferase NSD3, also known as Nuclear SET domain-containing protein 3, Protein whistle, WHSC1-like 1 isoform 9 with methyltransferase activity to lysine or Wolf-Hirschhorn syndrome candidate 1-like protein 1, (WHSC1-like protein 1), and encoded by the gene WHSC1L1/NSD3, is a histone methylase that preferentially methylates Lys-4 and Lys-27 of histone H3. Lys-4 methylation is generally a specific tag for transcriptional activation while Lys-27 methylation is a mark for transcriptional repression. NSD3 is highly expressed in many tissues including the brain, heart and skeletal muscle. Overexpression of NSD3 is associated with certain forms of lung cancer as well as certain other cancers. NSD3 also facilitates the expression of many other genes important in cancer progression such as Brd4. Brd4 through its extraterminal domain interacts with NSD3 which regulates transcription by modifying the chromatin microenvironment at Brd4 target genes.

Especificidade

This antibody is expected to cross react with isofomrs 1, 2, and 4 based upon immunogen design.

Imunogênio

KLH-conjugated linear peptide corresponding to a region near the N-terminus of human Histone-lys N-methyltransferase NSD3.

Aplicação

Detect Histone-lysine N-methyltransferase NSD3 using this rabbit polyclonal antibody, Anti-Histone-lysine N-methyltransferase NSD3 Antibody validated for use in western blotting & IHC.

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Histone-lys N-methyltransferase NSD3 in human and mouse cerebellum tissues.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Qualidade

Evaluated by Western Blotting in BALB/3T3 nuclear extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected Histone-lys N-methyltransferase NSD3 in 10 µg of BALB/3T3 nuclear extract.

Descrição-alvo

~180 kDa, ~160 kDa, ~90 kDa, and ~70 kDa observed. Uniprot desribes four isoforms at 162 kDa (isoform 1), 156 kDa (isoform 2), 73 kDa (isoform 3), and isoform 4 (155 kDa). This protein is known to be acetylated and phosphorylated and may be observed at higher molecular weights. Uncharacterized band(s) may be observed in some cell lysates.

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable