AB5820
Anti-Syntaxin 1 Antibody
Chemicon®, from rabbit
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Preço e disponibilidade não estão disponíveis no momento.
fonte biológica
rabbit
forma do anticorpo
affinity purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
purificado por
affinity chromatography
reatividade de espécies
mouse, rat
fabricante/nome comercial
Chemicon®
técnica(s)
immunohistochemistry: suitable
western blot: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... STX1A(6804)
Especificidade
Recognizes Syntaxin 1A and Syntaxin 1B. The epitope does not share homology with any other known proteins.
Imunogênio
GST fusion protein corresponding to a cytoplasmic part of rat Syntaxin 1A (Accession number P32851) (Bennet et al. 1992; Inoue & Akagawa 1992).
Aplicação
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
This Anti-Syntaxin 1 Antibody is validated for use in WB, IH for the detection of Syntaxin 1.
Western blot: 1:700-1:1,250 (0.8-1.5 g/mL) using ECL on rat brain membranes.
Immunohistochemistry: AB5820 is directed against an intracellular epitope. Thus, a procedure including permeabilization of cells with 0.2% Triton X 100 is recommended. It is recommended that you start with a working concentration of 30 μg/mL (1:33).
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: AB5820 is directed against an intracellular epitope. Thus, a procedure including permeabilization of cells with 0.2% Triton X 100 is recommended. It is recommended that you start with a working concentration of 30 μg/mL (1:33).
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.
forma física
Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
Armazenamento e estabilidade
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Nota de análise
Control
CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 mL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 5 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 mL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 5 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Frases de perigo
Declarações de precaução
Classificações de perigo
Aquatic Chronic 3
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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