AB3879
Anti-Acetyl Lysine Antibody
Chemicon®, from rabbit
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
affinity isolated antibody
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
purificado por
affinity chromatography
reatividade da espécie (prevista por homologia)
all
fabricante/nome comercial
Chemicon®
técnica(s)
ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Condições de expedição
wet ice
modificação pós-traducional do alvo
acetylation (Lys)
Descrição geral
In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.
Especificidade
Recognizes proteins acetylated on lysine residues. This antibody recognizes multiple acetylated proteins including acetylated histones, acetylated BSA, and acetylated MBP, and exhibits no reaction with non-acetylated proteins.
Imunogênio
Acetylated KLH.
Aplicação
Anti-Acetyl Lysine Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Acetyl Lysine & has been validated in ELISA, IF, IHC, IP & WB.
Immunoprecipitation:
A previous lot was used at 10 µg/mg crude protein.
ELISA:
0.25-1 μg/mL of a previous lot was used.
Immunohistochemistry:
2.5 μg/mL of a previous lot was used.
Immunofluorescence:
5 μg/mL of a previous lot was used.
Optimal working dilutions must be determined by end user.
A previous lot was used at 10 µg/mg crude protein.
ELISA:
0.25-1 μg/mL of a previous lot was used.
Immunohistochemistry:
2.5 μg/mL of a previous lot was used.
Immunofluorescence:
5 μg/mL of a previous lot was used.
Optimal working dilutions must be determined by end user.
Research Category
Signaling
Epigenetics & Nuclear Function
Signaling
Epigenetics & Nuclear Function
Research Sub Category
General Post-translation Modification
Histones
General Post-translation Modification
Histones
Qualidade
Routinely evaluated by Western Blot on Hela Acid Extract/Sodium Butyrate lysates.
Western Blot Analysis: 1:500 dilution of this lot detected lysine acylated proteins on 10 μg of Hela Acid Extract/Sodium Butyrate lysates.
Western Blot Analysis: 1:500 dilution of this lot detected lysine acylated proteins on 10 μg of Hela Acid Extract/Sodium Butyrate lysates.
forma física
ImmunoAffinity Purified
Purified rabbit polyclonal in buffer containing PBS with 50% glycerol.
Armazenamento e estabilidade
Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Nota de análise
Control
TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Annamaria Sandomenico et al.
mAbs, 8(8), 1575-1589 (2016-10-30)
Post-translational modifications (PTMs) strongly influence the structure and function of proteins. Lysine side chain acetylation is one of the most widespread PTMs, and it plays a major role in several physiological and pathological mechanisms. Protein acetylation may be detected by
PSG gene expression is up-regulated by lysine acetylation involving histone and nonhistone proteins.
Camolotto, SA; Racca, AC; Ridano, ME; Genti-Raimondi, S; Panzetta-Dutari, GM
Testing null
Niek G J Leus et al.
Biochemical pharmacology, 108, 58-74 (2016-03-20)
The increasing number of patients suffering from chronic obstructive pulmonary disease (COPD) represents a major and increasing health problem. Therefore, novel therapeutic approaches are needed. Class I HDACs 1, 2 and 3 play key roles in the regulation of inflammatory
Lorenzo Schiaffino et al.
Scientific reports, 8(1), 12875-12875 (2018-08-29)
Dysregulation in acetylation homeostasis has been implicated in the pathogenesis of the amyotrophic lateral sclerosis (ALS), a fatal neurodegenerative disorder. It is known that the acetylation of transcriptional factors regulates their activity. The acetylation state of NF-kB RelA has been
Terry R Medler et al.
Molecular cancer research : MCR, 14(10), 994-1008 (2016-07-01)
Stat5a is a transcription factor utilized by several cytokine/hormone receptor signaling pathways that promotes transcription of genes associated with proliferation, differentiation, and survival of cancer cells. However, there are currently no clinically approved therapies that directly target Stat5a, despite ample
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