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70236-M

Sigma-Aldrich

BL21(DE3)pLysS Singles Competent Cells - Novagen

Escherichia coli, rod shaped

Sinônimo(s):

BL21(DE3) competent cells, Competent bacterial cells

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About This Item

Código UNSPSC:
41106202
NACRES:
NA.54
Preço e disponibilidade não estão disponíveis no momento.

Nome do produto

BL21(DE3)pLysS Singles Competent Cells - Novagen,

fonte biológica

Escherichia coli

Nível de qualidade

100

fabricante/nome comercial

Novagen®

condição de armazenamento

OK to freeze
avoid repeated freeze/thaw cycles

modo de crescimento

adherent or suspension

morfologia

rod shaped

técnica(s)

microbiological culture: suitable

transformação celular

transformation efficiency: >2×107 cfu/μg

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

Categorias relacionadas

Descrição geral

BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
Genotype: F-ompT hsdSB(rB- mB-) gal dcm (DE3) pLysS (CamR)

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Componentes

•11 or 22Singles Competent Cells•2 or 4 × 2 mlSOC Medium•10 µlTest Plasmid

Advertência

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Informações legais

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Certificados de análise (COA)

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Questions

1–2 of 2 Questions  

  1. How many µL are in each tube of the singles?

    1 answer

    1. Each reaction tube contains 50 ul of competent cells. This product is available as a set of 11 or 22 tubes or reactions.

      Helpful?

  2. What is the difference between the strain expressing pLysE and pLysS?

    1 answer

    1. Products 69451-M and 70236-M are exactly the same competent cells. The only difference is in the packaging. The 70236 is already in 50uL aliquots, which are single-use, each aliquot is used for one reaction. This reference is available in two sizes of 11 or 22 reactions. While product 69451 is available in single 0.4 or 1mL volumes which may be thawed once and dispensed to smaller aliquots.
      The difference between the 69451-M/70236-M products and the CMC0015 BL21 is the type of plasmid that they express for the T7 lysozymes. The expression of T7 lysozyme reduces the basal expression of the target genes, inhibiting the T7 RNA polymerase. Specifically, with the expression of the plasmid pLysE (Ref. CMC0015) a higher expression of the levels of T7 Lysozime is obtained in comparison with the plasmid pLysS (ref. 69451-M/70236-M). Consequently, with reference CMC0015 there is even less risk of basal expression of the target protein in the absence of IPTG stimulation. This could be particularly relevant for toxic proteins that could affect competent cell viability.

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