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407140

Sigma-Aldrich

Anti-IP₃ Receptor Mouse mAb (IPR.1)

liquid, clone IPR.1, Calbiochem®

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About This Item

Código UNSPSC:
12352203
Preço e disponibilidade não estão disponíveis no momento.

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified antibody

tipo de produto de anticorpo

primary antibodies

clone

IPR.1, monoclonal

Formulário

liquid

contém

≤0.1% sodium azide as preservative

reatividade de espécies

rabbit, human, canine, mouse, rat, bovine, porcine

fabricante/nome comercial

Calbiochem®

condição de armazenamento

OK to freeze
avoid repeated freeze/thaw cycles

Isotipo

IgG2b

Condições de expedição

wet ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... ITPR1(3708)
mouse ... Itpr1(16438)
rat ... Itpr1(25262)

Descrição geral

Purified mouse monoclonal antibody. Recognizes all of the ~260 kDa IP3 receptor subtypes.
Recognizes all of the ~260 kDa IP3 receptor subtypes.
This Anti-IP₃ Receptor Mouse mAb (IPR.1) is validated for use in ELISA, FC, IF, IHC, IP, Radioimmunoassay, Immunoblotting for the detection of IP₃ Receptor.

Imunogênio

a synthetic peptide (GGVGDVLRKPS) corresponding to amino acids near the C-terminus of the IP₃ receptor

Aplicação

ELISA (1:1000)

Flow Cytometry (1:500)

Immunofluorescence (1:100)

Immunohistochemistry (1:100; see application references)

Immunoprecipitation (1:100; see application references)

Radioimmunoassay (1:5000)

Immunoblotting (see application references)

Embalagem

Please refer to vial label for lot-specific concentration.

Advertência

Toxicity: Standard Handling (A)

forma física

In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.

Reconstituição

Following initial thaw, aliquot and freeze (-70°C).

Nota de análise

Positive Control
Lymphocytes, macrophages, granulocytes, fibroblasts, epithelial and edothelial cells, skeletal muscle, cardiac muscle and brain tissue

Outras notas

Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int.17, 751.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem.268, 7290.
Specific for the C-terminal cytoplasmic domain of the IP3 receptor. For immunohistochemistry, this antibody requires FITC and confocal microscopy. Variables associated with assay conditions will dictate the proper working dilution.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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L Cavallini et al.
The Journal of biological chemistry, 271(10), 5545-5551 (1996-03-08)
Prostaglandin I2 (PGI2) and sodium nitroprusside (SNP) induce a rapid decay of the thrombin-promoted increase of [Ca2+]i in aspirin-treated platelets incubated in the absence of external Ca2+. The mechanism of their effect was studied with a new method which utilizes
Xudong Feng et al.
Nature communications, 5, 4487-4487 (2014-07-30)
Inositol 1, 4, 5-trisphosphate receptor (IP3R)-mediated Ca(2+) release from the endoplasmic reticulum (ER) triggers many physiological responses in neurons, and when uncontrolled can cause ER stress that contributes to neurological disease. Here we show that the unfolded protein response (UPR)
L Lagostena et al.
The Journal of physiology, 531(Pt 3), 693-706 (2001-03-17)
1. Hensen's cells in the isolated cochlea were stimulated by extracellular adenosine 5'-triphosphate (ATP) applied to their endolymphatic surface while changes in membrane current and intracellular calcium concentration ([Ca2+]i) were measured simultaneously. The response consisted of (i) an initial rapid
John C Hennessey et al.
Pharmacology research & perspectives, 3(2), e00112-e00112 (2015-03-03)
Endothelial cell (EC)-dependent vasodilation by proteinase-activated receptor 2 (PAR2) is preserved in small caliber arteries in disease states where vasodilation by muscarinic receptors is decreased. In this study, we identified and characterized the PAR2-mediated intracellular calcium (Ca(2+))-release mechanisms in EC
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Journal of cell science, 133(13) (2020-06-18)
The role of two-pore channel type 2 (TPC2, encoded by tcpn2)-mediated Ca2+ release was recently characterized in zebrafish during establishment of the early spinal circuitry, one of the key events in the coordination of neuromuscular activity. Here, we extend our

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