14-760-M

Ret (V804M) Protein, active, 10 µg

Active, N-Terminal GST-tagged, recombinant, human Ret amino acids 658 – end containing the V804M mutation. For use in Kinase Assays.

• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Propriedades

• fonte biológica: human
• Nível de qualidade: 100; 300
• recombinante: expressed in baculovirus infected Sf21 cells
• peso molecular: M_w 79.2 kDa
• fabricante/nome comercial: Upstate^®
• técnica(s): activity assay: suitable (kinase)
• nº de adesão NCBI: NM_000323
• nº de adesão UniProt: P07949; P35546 (mouse)
• Informações sobre genes: human ... RET(5979)

Descrição

Descrição geral

N-Terminal GST-tagged, recombinant, human Ret amino acids 658 – end containing the V804M mutation.

Product Source: Expressed by baculovirus in Sf21 insect cells.

The V804M mutation has been identified in patients with familial medullary thyroid carcinoma (FMTC) (Fink et al, (1996), Int. J. Cancer, 69, 312-316; Lesueur F. et al,(2005), JCEM, 90, 3454-3457).

Aplicação

Research Category
Inflammation & Immunology

Ações bioquímicas/fisiológicas

Protein Target: Ret

Target Sub-Family: TK

Embalagem

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-760M when ordering the 250 µg size.

Qualidade

Routinely evaluated by phosphorylation of CHKtide.

forma física

50mM Tris-HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Glutathione agarose affinity chromatography

Armazenamento e estabilidade

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Outras notas

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H317
• Declarações de precaução: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classificações de perigo: Skin Sens. 1
• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable