14-752-M

GRK7 Protein, active, 10 µg

• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Propriedades

• fonte biológica: human
• Nível de qualidade: 100; 300
• peso molecular: M_w 66 kDa
• fabricante/nome comercial: Upstate^®
• técnica(s): activity assay: suitable (kinase)
• nº de adesão NCBI: NM_139209.2
• nº de adesão UniProt: Q8WTQ7

Descrição

Descrição geral

N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells.

Recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells.

Aplicação

Active, N-terminal 6His-tagged, recombinant, full length human GRK7 expressed by baculovirus in Sf21 cells. For use in Kinase Assays.

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease|Metabolic Disorders

Ações bioquímicas/fisiológicas

• Protein Target: GRK7
• Target Sub-Family:AGC

Qualidade

Routinely evaluated by phosphorylation of 2 mg/mL casein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of GRK7.

Definição da unidade

Specific Activity: where one unit of GRK7 activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30°C with a final ATP concentration of 100μM.

forma física

10μg of recombinant protein in 100 μl of 50 mM Tris-HCl pH 8.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Format: Purified using Ni²⁺/NTA agarose. Purity 99% by SDS-PAGE and Coomassie blue staining.

Armazenamento e estabilidade

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H317
• Declarações de precaução: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classificações de perigo: Skin Sens. 1
• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable