14-742-M

FGFR2 (N549H) Protein, active, 10 µg

Active, N-terminal, 6His-tagged, recombinant, human FGFR2 (N549H), amino acids 456-770. For use in Kinase Assays.

• Código UNSPSC: 12352202
• eCl@ss: 32160405
• NACRES: NA.32

Propriedades

• fonte biológica: human
• Nível de qualidade: 300
• recombinante: expressed in baculovirus infected Sf21 cells
• atividade específica: 6250 U/mg
• peso molecular: M_w 38.1 kDa
• fabricante/nome comercial: Upstate^®
• técnica(s): activity assay: suitable (kinase)
• nº de adesão NCBI: NM_000141.3; NM_022970.2
• nº de adesão UniProt: P21802
• Informações sobre genes: human ... FGFR2(2263)

Descrição

Descrição geral

N-terminal, 6His-tagged, recombinant, human FGFR2 (N549H), amino acids 456-770.

Product Source: recombinant, human FGFR2 (N549H), amino acids 456-770, expressed by baculovirus in Sf21 insect cells.

Aplicação

Research Category
Metabolism

Research Sub Category
Cardiovascular Disease

Metabolic Disorders

Ações bioquímicas/fisiológicas

Protein Target: FGFR2

Target Sub-Family: TK

Qualidade

Routinely evaluated by phosphorylation of 500 μM GGEEEEYFELVKKKK.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of active GFGR2 (N549H).

Definição da unidade

Specific Activity: where one unit of FGFR2 (N549H) activity is defined as 1nmol phosphate incorporated into 500μM GGEEEEYFELVKKKK per minute at 30°C with a final ATP concentration of 100µM.

forma física

10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Ni²⁺/NTA-agarose

Armazenamento e estabilidade

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Pictogramas: GHS07
• Palavra indicadora: Warning
• Frases de perigo: H317
• Declarações de precaução: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classificações de perigo: Skin Sens. 1
• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable