07-448-S

Anti-monomethyl-Histone H3 (Lys27) Antibody, Trial Size

Upstate^®, from rabbit

• Sinônimo(s): H3K27me1, Histone H3 (monomethyl K27), H3 histone family, member T, Histone 3, H3, Histone cluster 3, H3
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: human, vertebrates
• fabricante/nome comercial: Upstate^®
• técnica(s): ChIP: suitable (ChIP-seq); dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• Isotipo: IgG
• nº de adesão NCBI: NP_003484
• nº de adesão UniProt: Q16695
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: monomethylation (Lys27)
• Informações sobre genes: human ... HIST1H3F(8968)

Descrição

Descrição geral

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Especificidade

Broad species cross-reactivity expected, based on sequence homology.

Recognizes monomethylated histone H3 (Lys27), Mr 17 kDa.

Imunogênio

Epitope: Lys27

KLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKSA… in which meK corresponds to monomethyl-lysine at residue 27 of human histone H3.

Aplicação

Anti-monomethyl-Histone H3 (Lys27), Trial Size Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 monomethylated on lysine 27 (H3K27me1). This antibody has dot blot (DB) verified specificity and is validated in, ICC, WB, ChIP, ChIP-seq. Small pack size is ideal for screening.

Immunocytochemistry & Dot Blot:
Reported by an independent laboratory (Kohlmaier, A., 2004; Perez-Burgos, L., 2004; Peters, A. H., 2003).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Qualidade

Routinely evaluated by western blot acid in extracted proteins from Hela cells.

Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells.

Descrição-alvo

17 kDa

forma física

Format: Purified

Protein A purified

Purified rabbit IgG in buffer containing 0.01 M phosphate buffer, pH 7.6, 0.125 M NaCl, 0.05% sodium azide and 30% glycerol.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
HeLa acid extract

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable