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07-1218

Sigma-Aldrich

Anti-PP1γ1 Antibody

Upstate®, from rabbit

Sinônimo(s):

Protein phosphatase 1C catalytic subunit, Serine/threonine-protein phosphatase PP1-gamma catalytic subunit, Protein phosphatase 1, catalytic subunit, gamma isoform, Serine/threonine phosphatase 1 gamma

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

affinity purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

reatividade de espécies

bovine, mouse, rat, human

fabricante/nome comercial

Upstate®

técnica(s)

immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG

nº de adesão NCBI

nº de adesão UniProt

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... PPP1CC(5501)

Descrição geral

Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR.

Especificidade

Recognizes Protein Phosphatase 1γ1. No cross reactivity with other recombinant PP1 isoforms.

Imunogênio

Epitope: C-terminus
The immunogen used to the purified peptide conjugated to KLH corresponding to the to the C –terminus of PP1 gamma 1 catalytic subunit (amino acids 311-323).

Aplicação

Anti-PP1γ1 Antibody detects level of PP1γ1 & has been published & validated for use in WB & IP.
Immunoprecipitation: A previous lot of this antibody was used on Immunoprecipitation.
Research Category
Signaling
Research Sub Category
Kinases & Phosphatases

Qualidade

Routinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PP-1 GAMMA on 10 μg of HeLa lysates.

Descrição-alvo

~37 kDa

Ligação

Replaces: AB4084

forma física

Format: Purified
Purified
Purified rabbit polyclonal IgG provided as 0.2µm sterile filtered solution in phosphate buffered saline with 0.08% sodium azide.

Armazenamento e estabilidade

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análise

Control
HeLa Cell Lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações legais

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 2

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Benjamin Combs et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 41(45), 9431-9451 (2021-10-06)
Pathologic tau modifications are characteristic of Alzheimer's disease and related dementias, but mechanisms of tau toxicity continue to be debated. Inherited mutations in tau cause early onset frontotemporal lobar dementias (FTLD-tau) and are commonly used to model mechanisms of tau
Bisrat T Woldemichael et al.
Nature communications, 7, 12594-12594 (2016-08-26)
Memory formation is a complex cognitive function regulated by coordinated synaptic and nuclear processes in neurons. In mammals, it is controlled by multiple molecular activators and suppressors, including the key signalling regulator, protein phosphatase 1 (PP1). Here, we show that
Julia Häfner et al.
Nature communications, 5, 4397-4397 (2014-07-23)
Upon congression at the spindle equator, vertebrate chromosomes display oscillatory movements which typically decline as cells progress towards anaphase. Kinesin-8 Kif18A has been identified as a suppressor of chromosome movements, but how its activity is temporally regulated to dampen chromosome
Pragya Sharma et al.
Diabetes, 65(9), 2606-2617 (2016-06-02)
Akt substrate of 160 kDa (AS160) phosphorylation on Thr(642) and Ser(588) by Akt is essential for insulin's full effect on glucose transport. However, protein phosphorylation is determined by the balance of actions by kinases and phosphatases, and the specific phosphatase(s)
Xiaoming Zhou et al.
American journal of physiology. Renal physiology, 305(3), F362-F369 (2013-05-31)
Activation of the transcription factor NFAT5 by high NaCl involves changes in phosphorylation. By siRNA screening, we previously found that protein targeting to glycogen (PTG), a regulatory subunit of protein phosphatase1 (PP1), contributes to regulation of high NaCl-induced NFAT5 transcriptional

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