806005

APN-Amine HCl

95%

• Sinônimo(s): 3-(4-(4-(Aminomethyl)-1H-1,2,3-triazol-1-yl)phenyl)propiolonitrile hydrochloride
• Fórmula empírica (Notação de Hill): C₁₂H₁₀N₅Cl
• Peso molecular: 259.69
• Código UNSPSC: 12352200
• ID de substância PubChem: 329768982

Propriedades

• Nível de qualidade: 100
• Ensaio: 95%
• Formulário: powder
• adequação da reação: reagent type: cross-linking reagent
• temperatura de armazenamento: 2-8°C
• cadeia de caracteres SMILES: N#CC#CC1=CC=C(N2C=C(CN)N=N2)C=C1.[H]Cl
• InChI: 1S/C12H9N5.ClH/c13-7-1-2-10-3-5-12(6-4-10)17-9-11(8-14)15-16-17;/h3-6,9H,8,14H2;1H
• chave InChI: JZGAUVVBWJHLGN-UHFFFAOYSA-N

Descrição

Aplicação

APN-Amine is a bifunctional crosslinker for thiol-to-carboxyl coupling. The coupling can be performed with high selectivity in biological medium using mild reaction conditions. The amino group readily reacts with activated carboxylic acids (e.g. NHS-esters) in basic conditions to produce stable amide bonds. The APN group can be used for coupling with free thiols (e.g. labeling of free cysteines in proteins).

Outras notas

Standard protein labeling procedure (cysteine labeling):

• Dissolve the protein in the appropriate buffer* with pH 6.5-9.0 (e.g. PBS) at 1-10 mg/mL concentration.
• Apply the appropriate amount of the stock solution of the reagent (1-5 molar eq. per free cysteine residue).
• Incubate at room temperature for 2 hours.
• If necessary, purify the protein conjugate using size exclusion chromatography or ultrafiltration.

*Note: avoid thiol-containing buffers.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable