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803618

Sigma-Aldrich

DTME (dithio-bis-maleimidoethane)

Sinônimo(s):

1,1′-(dithiodi-2,1-ethanediyl)bis-1H-Pyrrole-2,5-dione, 1-[2-[[2-(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]dithio]ethyl]-1H-pyrrole-2,5-dione, DTME

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About This Item

Fórmula empírica (Notação de Hill):
C12H12N2S2O4
Número CAS:
71865-37-7
Peso molecular:
312.36
Número MDL:
MFCD01863385
Código UNSPSC:
12352200
ID de substância PubChem:
329768821
NACRES:
NA.22

Ensaio

≥90%

forma

powder

peso molecular

312.37

adequação da reação

reagent type: cross-linking reagent

condição de armazenamento

desiccated

solubilidade

DMSO or DMF: soluble

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

O=C(C=CC1=O)N1CCSSCCN2C(C=CC2=O)=O

InChI

1S/C12H12N2O4S2/c15-9-1-2-10(16)13(9)5-7-19-20-8-6-14-11(17)3-4-12(14)18/h1-4H,5-8H2

chave InChI

SGVWDRVQIYUSRA-UHFFFAOYSA-N

Descrição geral

DTME is a homobifunctional, maleimide crosslinker for conjugation between sulfhydryl groups (-SH). Such bismaleimide crosslinkers are commonly used to explore and characterize protein structure (i.e., oligomerization) or protein interactions. DTME is similar in length to BMH but differs in containing a disulfide bond in its spacer arm, allowing crosslinks with DTME to be cleaved with reducing agent such as dithiothreitol (DTT).

Características e benefícios

  • Reactive groups: maleimide (both ends)
  • Reactive towards: sulfhydryl groups
  • Long (13.3A), cleavable, sulfhydryl-to-sulfhydryl crosslinker, composed of maleimide groups and 11-atom disulfide spacer arm
  • Water-insoluble—dissolve first in DMF or DMSO, then add to aqueous reaction buffers
  • Cleavable by reduction of disulfide spacer arm with DTT, TCEP or other reducing agent

Atenção

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

Pictogramas

Exclamation mark
GHS07

Palavra indicadora

Warning

Classificações de perigo

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Sigma-Aldrich

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