767964
Cell Free Amino Acid Mixture - 13C,15N
5-100 mM in water, 98 atom % 15N, 98 atom % 13C
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About This Item
Código UNSPSC:
12352209
NACRES:
NA.12
Preço e disponibilidade não estão disponíveis no momento.
pureza isotópica
98 atom % 13C
98 atom % 15N
Formulário
liquid
concentração
5-100 mM in water
técnica(s)
bio NMR: suitable
protein expression: suitable
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Categorias relacionadas
Aplicação
This product suitable as source of Stable Isotope Labeled amino acids for Cell Free Protein Synthesis. For Mass Spec applications, consider product 909653, Stable Isotope Labeled Amino Acid Mixture for Mass Spec.
The product may appear turbid, due to the relatively high concentration of tyrosine. Once diluted for use, solution should be clear.
The product may appear turbid, due to the relatively high concentration of tyrosine. Once diluted for use, solution should be clear.
Embalagem
1 mL aqueous solution of all 20 amino acids.
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.
Nota de análise
Amino acid concentrations vary by lot. Please refer to the specific lot Certificate of Analysis.
Approximate concentrations (mM):
ASP 60
THR 35
SER 35
GLU 40
PRO 20
GLY 100
ALA 100
VAL 40
MET 10
ILE 30
LEU 45
TYR 10
PHE 16
HIS 5
LYS 15
ARG 10
GLN 20
ASN 20
TRP 20
CYS 20
Approximate concentrations (mM):
ASP 60
THR 35
SER 35
GLU 40
PRO 20
GLY 100
ALA 100
VAL 40
MET 10
ILE 30
LEU 45
TYR 10
PHE 16
HIS 5
LYS 15
ARG 10
GLN 20
ASN 20
TRP 20
CYS 20
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2 - Skin Irrit. 2
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Zhenzhen Liu et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1124, 72-81 (2019-06-10)
A simple and fast LC-MS/MS method was developed and validated for simultaneous quantification of 20 proteinogenic l-amino acids (AAs) in a small volume (5 μL) of mouse plasma. Chromatographic separation was achieved on an Intrada Amino Acid column within 13 min via
Estelle Couradeau et al.
Nature communications, 10(1), 2770-2770 (2019-06-27)
The ability to link soil microbial diversity to soil processes requires technologies that differentiate active microbes from extracellular DNA and dormant cells. Here, we use BONCAT (bioorthogonal non-canonical amino acid tagging) to measure translationally active cells in soils. We compare
Preparation of amino acid mixtures for cell-free expression systems
Caschera, F., Noireaux, V
Biotechniques, 58, 40-43 (2015)
Filippo Caschera et al.
BioTechniques, 58(1), 40-43 (2015-01-22)
Here we present a procedure for preparing amino acid mixtures--having both the desired composition and a physiological pH--at high concentrations for cell-free expression systems. Up to 2.1 mg/mL of active protein was synthesized in batch mode reactions with an all
Sara Calhoun et al.
Communications biology, 4(1), 333-333 (2021-03-14)
Microalgae efficiently convert sunlight into lipids and carbohydrates, offering bio-based alternatives for energy and chemical production. Improving algal productivity and robustness against abiotic stress requires a systems level characterization enabled by functional genomics. Here, we characterize a halotolerant microalga Scenedesmus
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