PROBES
Custom qPCR Probes
Design and Order DNA, Locked Nucleic Acid, and BHQ Probes for qPCR
Sinônimo(s):
BHQ, BHQ Probes, Black Hole Quencher, Locked Nucleic Acid probes, Probes, Real-Time PCR Probes, locked nucleic acid, qPCR assays, qPCR designs
About This Item
Produtos recomendados
Dual-Labeled Probes
- Amounts: 1, 3, 5, and 10 OD
- Purification: HPLC
- Sequence Lengths: 15 - 40 bases
- Modifications: Locked Nucleic Acid available
- Dyes: Comprehensive offering of reporters and quenchers
- Quality Control: 100% mass spectrometry
- Format: Supplied dry in amber tubes
- Learn more
Molecular Beacons
- Amounts: 1, 3, 5, and 10 OD
- Purification: HPLC
- Sequence Lengths: 15 - 40 bases
- Modifications: Locked Nucleic Acid available
- Dyes: Comprehensive offering of reporters and quenchers
- Quality Control: 100% mass spectrometry
- Format: Supplied dry in amber tubes
- Learn more
LightCycler® Probes
- Amounts: 0.1, 0.25, 1.5, and 15 OD
- Purification: HPLC
- Sequence Lengths: 15 - 40 bases
- Modifications: Locked Nucleic Acid available
- Dyes: Probe 1 fluorescein (3′ end); Probe 2 phosphate (3′ end) and LC Red 610, 640, 670 or 705 (5′ end)
- Quality Control: 100% mass spectrometry
- Format: Supplied dry in amber tubes
- Learn more
Scorpions® Probes
- Amounts: 1, 5, and 5 OD
- Purification: HPLC
- Sequence Lengths: 30 - 60 bases
- Modifications: Locked Nucleic Acid available
- Dyes: Comprehensive offering of reporters and quenchers
- Quality Control: 100% mass spectrometry
- Format: Supplied dry in amber tubes
- Learn more
Locked Nucleic Acid
- Available for all Custom qPCR Probe Types
- Increased thermal stability and hybridization specificity
- Easier and more specific designs for problematic target sequences
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Black Hole Quencher™ (BHQ™)
- Available for all Custom qPCR Probe types
- No native fluorescence (emits heat instead of light), resulting in lower background fluorescence
- Increased signal-to-noise ratio, providing higher sensitivity
- Enables a wider choice of reporters for multiplexing
- Learn more
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Learn more about how digital PCR (dPCR) is used for absolute quantification and for analysis of minority sequences.
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LightCycler probes are highly sensitive and sequence-specific fluorescent probes designed for use with the Roche LightCycler instruments.
If laboratory handling error has been ruled out, contaminated primers and probes may be to blame for a signal from the qPCR no template control.
Licenças de marca para nossos produtos de oligonucleotídeos, inclusive Black Hole Quencher®, BlackBerry® Quencher e Locked Nucleic Acid (LNA®).
We offer an easy-to-use Oligo Analyzer tool, OligoEvaluator™ that provides better primer dimer analysis values for PCR, and serves as an online oligonucleotide sequence calculator and Tm calculator.
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