Pular para o conteúdo
Merck
Todas as fotos(1)

Documentos Principais

PROBES

Custom qPCR Probes

Design and Order DNA, Locked Nucleic Acid, and BHQ Probes for qPCR

Sinônimo(s):

BHQ, BHQ Probes, Black Hole Quencher, Locked Nucleic Acid probes, Probes, Real-Time PCR Probes, locked nucleic acid, qPCR assays, qPCR designs

Faça loginpara ver os preços organizacionais e de contrato


About This Item

Código UNSPSC:
41105904


Dual-Labeled Probes

  • Amounts: 1, 3, 5, and 10 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Comprehensive offering of reporters and quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more

Molecular Beacons

  • Amounts: 1, 3, 5, and 10 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Comprehensive offering of reporters and quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more

LightCycler® Probes

  • Amounts: 0.1, 0.25, 1.5, and 15 OD
  • Purification: HPLC
  • Sequence Lengths: 15 - 40 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Probe 1 fluorescein (3′ end); Probe 2 phosphate (3′ end) and LC Red 610, 640, 670 or 705 (5′ end)
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more

Scorpions® Probes

  • Amounts: 1, 5, and 5 OD
  • Purification: HPLC
  • Sequence Lengths: 30 - 60 bases
  • Modifications: Locked Nucleic Acid available
  • Dyes: Comprehensive offering of reporters and quenchers
  • Quality Control: 100% mass spectrometry
  • Format: Supplied dry in amber tubes
  • Learn more

Locked Nucleic Acid

  • Available for all Custom qPCR Probe Types
  • Increased thermal stability and hybridization specificity
  • Easier and more specific designs for problematic target sequences
  • Learn more

Black Hole Quencher™ (BHQ™)

  • Available for all Custom qPCR Probe types
  • No native fluorescence (emits heat instead of light), resulting in lower background fluorescence
  • Increased signal-to-noise ratio, providing higher sensitivity
  • Enables a wider choice of reporters for multiplexing
  • Learn more
Faça login para continuar

Para continuar lendo, faça login ou crie uma conta.

Ainda não tem uma conta?
Ana F Almeida-Santos et al.
Scientific reports, 9(1), 473-473 (2019-01-27)
The absence of companion may jeopardize mental health in social animals. Here, we tested the hypothesis that social isolation impairs social recognition memory by altering the excitability and the dialog between the olfactory bulb (OB) and the dorsal hippocampus (dHIP).
Rajeev Gupta et al.
Cell stem cell, 26(4), 527-541 (2020-03-21)
Umbilical cord blood (UCB) has had considerable impact in pediatric stem cell transplantation, but its wider use is limited in part by unit size. Long-term ex vivo culture offers one approach to increase engraftment capacity by seeking to expand stem and

Artigos

Learn more about how digital PCR (dPCR) is used for absolute quantification and for analysis of minority sequences.

Conteúdo relacionado

LightCycler probes are highly sensitive and sequence-specific fluorescent probes designed for use with the Roche LightCycler instruments.

If laboratory handling error has been ruled out, contaminated primers and probes may be to blame for a signal from the qPCR no template control.

Licenças de marca para nossos produtos de oligonucleotídeos, inclusive Black Hole Quencher®, BlackBerry® Quencher e Locked Nucleic Acid (LNA®).

We offer an easy-to-use Oligo Analyzer tool, OligoEvaluator™ that provides better primer dimer analysis values for PCR, and serves as an online oligonucleotide sequence calculator and Tm calculator.

Ver tudo

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica