TOSRTRO
Roche
Transcriptor One-Step RT-PCR Kit
sufficient for 50 reactions, sufficient for 150 reactions, suitable for RT-qPCR, suitable for RT-PCR, hotstart
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R$ 1.363,00
Previsão de entrega em21 de abril de 2025
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About This Item
R$ 1.363,00
Previsão de entrega em21 de abril de 2025
Produtos recomendados
uso
sufficient for 150 reactions
sufficient for 50 reactions
Nível de qualidade
Características
hotstart
fabricante/nome comercial
Roche
embalagem
pkg of 150 reactions (04655885001)
pkg of 50 reactions (04655877001)
técnica(s)
RT-PCR: suitable
RT-qPCR: suitable
entrada
purified RNA
método de detecção
probe-based
Descrição geral
The Transcriptor One-Step RT-PCR Kit offers the outstanding benefits of Transcriptor Reverse Transcriptase, as well as the advantages of a hot start one-step RT-PCR format.
The kit′s RT-PCR Enzyme Mix contains four different enzymes: Transcriptor Reverse Transcriptase ensures sensitive and robust reverse transcription with high yield; Protector RNase Inhibitor, fully active at elevated temperatures, provides maximum template protection during reverse transcription; and the Expand System – a blend consisting of Taq DNA Polymerase and a proofreading polymerase – minimizes the possibility of mutations, offering high yield and fidelity in the PCR.
The optimized RT-PCR Reaction Buffer includes high quality dNTPs and provides the overall improved performance of a hot start system. The buffer′s unique hot start component binds and sequesters primers at lower temperatures to prevent the primers from binding to nonspecific sites. Another component binds magnesium, in a temperature-dependent manner, to prevent uncontrolled DNA synthesis. This new buffer formulation is effective during reverse transcription as well as PCR, resulting in increased specificity and sensitivity.
The combination of optimized reaction buffer and enzyme mix ensures efficient transcription of difficult templates with high secondary structure and GC content without increasing reaction temperature.
Aplicação
Featuring an innovative reaction buffer, the kit combines sensitive, high-yield reverse transcription with the improved performance of a hot start system for high-fidelity, high-yield amplification of a variety of templates, including GC-rich RNA.
The kit offers a convenient, highly sensitive one-step RT-PCR method to generate transcripts up to 6.5 kb for applications such as:
- Qualitative, semi-quantitative, or quantitative analysis of RNA transcription levels
- Determining the presence or absence of RNA templates
- Quantifying gene expression through RNA analysis (e.g., for virus-level quantification)
- Cloning of RNA up to 6.5 kb
The one-step format is convenient, reduces reaction-to-reaction variability, and prevents contamination by minimizing hands-on steps.
Características e benefícios
- Obtain high sensitivity and yield in a wide amplification range:
- Achieve high specificity and reduce primer-dimers
- Produce long fragments:
- Transcribe a variety of templates, even the most difficult (e.g., GC-rich RNA):
- Save time and improve results with a convenient kit:
- Protect your RNA sample:
- Increase confidence in your results:
Componentes
- RT-PCR Enzyme Mix containing Transcriptor Reverse Transcriptase, Protector RNase Inhibitor, and the Expand System
- RT-PCR Reaction Buffer containing hot start mediating components and dNTPs – 5x concentrated
- Water, PCR Grade
- Control RNA – HAV RNA, in vitro transcript *
- Control primer mix HAV RNA (forward and reverse primer) *
* Included only in the 50-reaction pack size.
Qualidade
In addition, RT-PCR is performed on a dilution series of human liver total RNA (10 ng, 1 ng, and 0.1 ng). RT-PCR with specific primers for a 2.5 kb fragment of ApoB is performed using the standard RT-PCR protocol (reverse transcription at +50°C for 30 minutes) provided in the package insert. With 1 ng of RNA a clearly visible band is obtained after agarose gel electrophoresis and ethidium bromide staining.
Outras notas
Frases de perigo
Declarações de precaução
Classificações de perigo
Aquatic Chronic 3
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
does not flash
Ponto de fulgor (°C)
does not flash
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