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HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (30 cm column)

HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (30 cm column) application for HPLC

Matériaux

Colonne analytique

Réf. du produit
Description
Tarif

Zenix® SEC-300 Gel Filtration Column, 3 μm

L × I.D. 15 cm × 7.8 mm, 3 μm particle size, stainless steel

Étalon

Réf. du produit
Description
Tarif

Thyroglobulin from bovine thyroid

powder, ≥90% (agarose gel electrophoresis)

Glucose-6-phosphate déshydrogénase from baker′s yeast (S. cerevisiae)

Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

Albumine de sérum bovin

lyophilized powder, ≥96% (agarose gel electrophoresis)

Ribonucléase A from bovine pancreas

for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

CONDITIONS

column

Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm, 300 Å particles (Z777028)

mobile phase

150 mM potassium phosphate, pH 6.8

flow rate

as indicated on figure

column temp.

ambient

detector

UV, 280 nm

injection

10 μL

sample

each 1 mg/mL except uracil, 2.5 mg/mL

Description

Informations légales

Zenix is a registered trademark of Sepax Technologies