ZRB1476

Anti-GRHL2 Antibody, clone 2N18 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : Grainyhead-like protein 2 homolog;Brother of mammalian grainyhead;Transcription factor CP2-like 3
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 2N18, recombinant monoclonal
• Description: recombinant, expressed in HEK 293 cells
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 71.11 kDa; observed mol wt ~75 kDa
• Produit purifié par: using Protein A
• Espèces réactives: rat, human, mouse
• Réactivité de l'espèce (prédite par homologie): monkey, feline, bovine, canine
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotype: IgG
• Séquence de l'épitope: Internal
• Numéro d'accès Protein ID: NP_079191
• Numéro d'accès UniProt: Q6ISB3
• Autre catégorie plus écologique: , Aligned
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spécificité

Clone 2N18 is a Rabbit recombinant monoclonal antibody that specifically detects Grainyhead-like protein 2 homolog (GRHL2). It targets an epitope within 15 amino acids from the internal region.

Immunogène

KLH-conjugated linear peptide corresponding to 15 amino acids from the internal region of human Grainyhead-like protein 2 homolog (GRHL2).

Application

Quality Control Testing

Evaluated by Western Blotting in Mouse testis tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GRHL2 in Mouse testis tissue lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GRHL2 in lysates from human testis tissue and KNRK cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GRHL2 in human skin tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected GRHL2 in lysates from A431, HeLa, HepG2, and NIH 3T3 cells.

Affinity Binding Assay: A representative lot of this antibody bound GRHL2 with a KD of 2.5 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Description de la cible

Grainyhead-like protein 2 homolog (UniProt: Q6ISB3; also known as Brother of mammalian grainyhead, Transcription factor CP2-like 3, GRHL2) is encoded by the GRHL2 (also known as BOM, TFCP2L3) gene (Gene ID: 79977) in human. GRHL2 is a homodimeric transcription factor that plays an important role in primary neurulation and in epithelial development. It can also exist in heterodimeric form with GRHL1 or GHRL3. It is also involved in wound healing, maintenance of epidermal integrity, and epithelial-to-mesenchymal transition. It binds directly to the consensus DNA sequence 5′-AACCGGTT-3′ acting as an activator and repressor on distinct target genes. It is present at cell-cell contact sites and its expression is observed to be high in keratinocytes, placenta, prostrate, brain, and kidney. It is expressed in proliferating cells and its expression is reduced during senescence. During embryogenesis, it plays unique and cooperative roles with GRHL3 in establishing distinct zones of primary neurulation. It is shown to be essential for closure 3 (rostral end of the forebrain), closure 2 (forebrain/midbrain boundary), and posterior neuropore closure. In keratinocytes, it plays a role in telomerase activation during cellular proliferation and regulates TERT expression by binding to TERT promoter region and inhibiting DNA methylation at the 5′-CpG island, possibly by interfering with DNMT1 enzyme activity. Overexpression of GRHL2 promotes cell growth and colony formation, and simultaneously suppresses cell migration in non-small cell lung carcinoma cells. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Pan, X., et al. (2017). Am. J. Transl. Res. 9(9); 4217- 4226; Chen, W., et al. (2010). J. Biol. Chem. 285 (52); 40852-40863).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 13 - Non Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable