T4532
Tryptose Broth
suitable for insect cell culture
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About This Item
Code UNSPSC :
41106507
Nomenclature NACRES :
NA.75
Produits recommandés
Source biologique
Porcine pancreas
Forme
liquid
Technique(s)
cell culture | insect: suitable
Conditions d'expédition
ambient
Température de stockage
2-8°C
Application
In addition to its use for the growth of fastidious micro-organisms, Tryptose Phospate Broth (TPB) has been studied as supplement for the preparation of media that supports vaccine production in BHK-21 cells and the growth of SF21 insect cells in high-density perfusion culture stirred-tank bioreactors.
Composants
Tryptose Phosphate Broth (TPB) is composed of four components: Tryptose (20g/L); Dextrose (2g/L); NaCl (5g/L) and Disodium Phosphate (2.5g/L) typically adjusted to pH 7.3. The tryptose component is a peptone (mixture of amino acids and short peptides) derived by the mixed enzymatic hydrolysis (pancreatic enzymes) of the milk protein casein. This hydrolysate provides a source of amino acid based nutrients and survival factors that support the growth of fastidious micro-organisms such as Brucella, Streptococcus, and Neisseria; as well as eukaryotic cells such as insect and animal cells. Dextrose provides a fermentable carbohydrate that can be used by fastidious mico-organisms. Sodium chloride maintains the osmotic and ionic equilibrium and disodium phosphate provides the basic buffering capacity.
Autres remarques
This Tryptose broth is closely related to Tryptose phosphate broth, but does not contain the phosphate component and it contains a lower concentration of dextrose.
Notes préparatoires
This formulation contains Tryptose (20 g/L); Dextrose (1 g/L); and NaCl (5g/L) adjusted to a final pH of 7.2.
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Souvent commandé avec ce produit
Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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Les clients ont également consulté
S M Deutschmann et al.
Enzyme and microbial technology, 16(6), 506-512 (1994-06-01)
Spodoptera frugiperda insect cells (IPLB-Sf21-AE) (Sf21), infected with baculovirus expression vectors during their exponential growth phase, are commonly used to produce a variety of heterologous recombinant proteins. In the present study the culture conditions of these insect cells were studied
S N Saha et al.
Vaccine, 7(4), 357-363 (1989-08-01)
Studies were undertaken to develop a cheaper medium with indigenous sources of peptone and casein hydrolysate for continuous culture of BHK-21 (suspension) cells and production of foot-and-mouth disease (FMD) vaccine. Eleven batches of experimental media were prepared using different indigenous
A I Josemans et al.
Annals of the New York Academy of Sciences, 969, 141-146 (2002-10-17)
The in vitro culture of Cowdria ruminantium, the causative agent of heartwater in domestic ruminants, was first achieved in 1985. Culture media were usually supplemented with serum and tryptose phosphate broth, both undefined components, contributing to great variability. Recently, we
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