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SRP0135

Sigma-Aldrich

G9a human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonyme(s) :

EHMT2, Euchromatic histone-lysine N-methyltransferase 2, HLA-B-associated transcript 8, KMT1C, Lysine N-methyltransferase 1C

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Source biologique

human

Produit recombinant

expressed in E. coli

Pureté

≥70% (SDS-PAGE)

Forme

aqueous solution

Poids mol.

74.6 kDa

Conditionnement

pkg of 50 μg

Concentration

>0.02 mg/mL

Numéro d'accès NCBI

NM_006709

Numéro d'accès UniProt

Q96KQ7

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... EHMT2(10919)

Description générale

Human G9a (euchromatic histone-lysine N-methyltransferase 2) with N-terminal GST tag, MW = 74.6 kDa, expressed in an E. coli expression system. G9a is a member of SET (Su(var)3-9, enhancer-of-zeste and trithorax) domain containing, Su(var)3-9 family of proteins. This domain is evolutionary conserved.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Actions biochimiques/physiologiques

The encoded protein G9a (euchromatic histone-lysine N-methyltransferase 2) is a methyltransferase that methylates lysine residues of histone H3. Overexpression of G9a is observed in cancer. It is known to participate in cancer cell migration, invasion and metastasis. G9a is an epigenetic regulator and can inhibit the action of tumor suppressor genes, thereby, contributes to carcinogenesis. Thus, G9a is considered to be an oncogenic and metastatic factor. It also affects DNA damage repair mechanism, which might make tumor cells resistant to radiation and chemotherapy. G9a serves as a target for cancer therapy including breast cancer.

Définition de l'unité

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Forme physique

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Notes préparatoires

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

G9a coordinates with the RPA complex to promote DNA damage repair and cell survival
Yang Q, et al.
Proceedings of the National Academy of Sciences of the USA, 114(30), E6054-E6063 (2017)
G9a drives hypoxia-mediated gene repression for breast cancer cell survival and tumorigenesis
Casciello F, et al.
Proceedings of the National Academy of Sciences of the USA, 114(27), 7077-7082 (2017)
H3K27me1 is essential for MMP-9-dependent H3N-terminal tail proteolysis during osteoclastogenesis
Kim K, et al.
Epigenetics & Chromatin, 11(1), 23-23 (2018)
SATB2 suppresses non-small cell lung cancer invasiveness by G9a
Ma Yi-nan, et al.
Clinical and Experimental Medicine, 18(1), 37-44 (2018)
G9a, a multipotent regulator of gene expression
Shankar S, et al.
Epigenetics, 8(1), 16-22 (2013)

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