Too much residual medium or cell mass may lead to recovery of some large RNAs. It is critical to remove as much residual medium as possible by re-centrifuging the pellet. One can also test different ratios of the lysis mix (Small RNA Lysis Buffer and Binding Solution).
SNC10
Mission® Small RNA Purification Kit
1 sufficient for 10 preparations
Synonyme(s) :
microRNA Isolation Kit
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102,00 €
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About This Item
Code UNSPSC :
41105501
Nomenclature NACRES :
NA.52
102,00 €
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
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Utilisation
1 sufficient for 10 preparations
Technique(s)
DNA extraction: suitable
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Catégories apparentées
Description générale
Sigma′s mirPremier microRNA Isolation Kit provides a rapid and efficient method for purifying and enriching miRNAs and other small RNAs from diverse biological sources, including mammalian cell cultures, animal tissues, plant tissues, and microbial cultures, without using hazardous organic extractions. microRNAs (miRNAs) are a class of small RNA molecules, about 21 nucleotides (nt) in length, that regulate gene expression in a variety of manners, including translational repression, mRNA cleavage and deadenylation. In addition, the kit also can be used for isolating total RNA if messenger RNA or other large RNAs are of interest.
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Application
Caractéristiques et avantages
- Developed to enhance the efficiency of isolating microRNA and other small RNA molecules directly from a wide range of biological samples.
- Facilitates rapid and effective extraction and concentration of miRNA in 30 minutes for downstream applications.
- Capable of extracting miRNA with high purity with no detectable large RNA.
- Hazardous organic extractions are not involved.
Informations légales
mirPremier is a registered trademark of Merck KGaA, Darmstadt, Germany
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Warning
Mentions de danger
Classification des risques
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
Code de la classe de stockage
10 - Combustible liquids
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Extraction of Small RNA and qPCR Validation of miRNAs in Vigna mungo
Paul S, et al.
Bio-protocol, 5(5), e1417-e1417 (2015)
Ying Zhang et al.
Genome biology, 18(1), 105-105 (2017-06-16)
Post-transcriptional regulation of gene expression can be achieved through the control of mRNA stability, cytoplasmic compartmentalization, 3' UTR length and translational efficacy. Spermiogenesis, a process through which haploid male germ cells differentiate into spermatozoa, represents an ideal model for studying
Xiaoqiong Tang et al.
Cancer management and research, 12, 2119-2125 (2020-04-14)
LncRNA mortal obligate RNA transcript (MORT) is downregulated in different types of cancer, indicating its involvement in cancer biology. In this study, MORT and miRNA-16 were both downregulated in plasma of mantle cell lymphoma (MCL) patients than that in the
MicroRNAs control mRNA fate by compartmentalization based on 3? UTR length in male germ cells
Zhang Y, et al.
Genome Biology, 18(1), 105-105 (2017)
Identification of microRNA-mRNA regulatory network associated with oxidative DNA damage in human astrocytes
Nwokwu CD, et al.
ASN Neuro, 14, 17590914221101704-17590914221101704 (2022)
Contenu apparenté
Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).
Sigma-Aldrich® Advanced Genomics est le premier fournisseur de technologies d'édition et d'extinction géniques, dont CRISPR, Cas9, les ARN guides synthétiques (ARNsg) et les nucléases à doigts de zinc (ZFN pour "Zinc Finger Nucleases").
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How can I avoid co-purifying mRNAs and rRNAs when purifying small RNAs from gram negative bacteria using mirPremier® microRNA Isolation Kit?
1 answer-
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Can I purify 18S and 28S large RNAs along with miRNAs with the mirPremier® microRNA Isolation Kit?
1 answer-
Large RNAs (18S and 28S) from the pellet fraction can be purified after transferring the microRNA-containing supernatant to a new tube by using the total RNA protocol or by phenol/chloroform extraction.
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Has the mirPremier® microRNA Isolation Kit been tested on sperm cells?
1 answer-
We have not tested mirPremier™ microRNA Isolation Kit with sperm cells.
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Can the mirPremier® microRNA Isolation Kit be used to isolate small RNAs from purified total RNA?
1 answer-
We have used to the kit to purify in vitro transcribed small RNAs with great success, but have not done so with purified total RNA. Here are the steps we would recommend trying with purified total RNA:1. Prepare a lysis mix with 0.7 vol. Small RNA Lysis Buffer (M1070) and 0.3 vol. Binding Solution (L8042).2. Add 500 ul of lysis mix with 50 ul total RNA and mix thoroughly.3. Spin at 14000 rpm for 5 min to precipitate large RNA.5. Transfer the supernatant to a new tube.6. Add 610 ul (1.1 vol.) 100% ethanol to the supernatant and mix well.7.Transfer the mixture to a binding column and spin 1 min to bind. Repeat the binding step with the remaining mixture.8. Wash the column first with 700 ul 100% ethanol, and then with ethanol-diluted wash Solution 2.9. Dry the column and elute small RNA.
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