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SHM03

Sigma-Aldrich

Billes MISSION® ExpressMag®

Increases transduction efficiency

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100 μL
238,00 €
300 μL
476,00 €
1 ML
1 330,00 €
5 ML
6 050,00 €
10 ML
11 460,00 €

238,00 €

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Toutes les photos(1)

Sélectionner une taille de conditionnement

Changer de vue
100 μL
238,00 €
300 μL
476,00 €
1 ML
1 330,00 €
5 ML
6 050,00 €
10 ML
11 460,00 €

About This Item

Code UNSPSC :
41106609
Nomenclature NACRES :
NA.51

238,00 €

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en grosDemander des informations complémentaires

Niveau de qualité

200

Gamme de produits

MISSION®

Durée de conservation

3 yr

Température de stockage

2-8°C

Description générale

To view protocols from Sigma collaborators for ExpressMag system visit the link below.
www.sigmaaldrich.com/mission-protocols

Application

When coupled with MISSION® ExpressMag® magnetics, ExpressMag beads will increase the transduction efficiency of lentiviral particles. Increases in transduction efficiency are particularly striking in primary cells and in suspension cells.

Notes préparatoires

Do NOT FREEZE! Significant decrease in efficacy will occur if ExpressMag bead solution is frozen.

Informations légales

ExpressMag is a registered trademark of Sigma-Aldrich Co. LLC
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
0000284892
0000284891
0000284891
SLCQ7610
SLCQ8535

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Questions

1–6 of 6 Questions  

  1. What is the typical fold increase in transduction efficiency when using ExpressMag beads?

    1 answer

    1. The transduction efficiency increase observed when using the ExpressMag products will depend on the cell line. It is reasonable to expect a roughly 10-fold or greater increase.

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  2. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  4. What other viruses have been successfully introduced into cells via the use of ExpressMag beads?

    1 answer

    1. Adenovirus, SIV, Retrovirus, Measles virus and HIV have all been successfully introduced into cells using ExpressMag.

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  5. What MISSION products have been successfully introduced into cells using ExpressMag® beads?

    1 answer

    1. MISSION Lentiviral Particles (various targets/clones), SHC002V, SHC003V and SHC008V have been successfully introduced into cells using ExpressMag beads.

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  6. What cell lines have been transduced successfully using ExpressMag Beads?

    1 answer

    1. The following cell types have been successfully transduced using ExpressMag Beads: PBL, PBMC, T Cells, GMB primary tumor cells, A549, NYGM, T98G, U251, U87,YH-13, YK6-1, KS-1, NIH3T3, K562, Vero, HeLa, CHO, L929, B95a, H9, HUVEC, CD4+ Primary Human T-Cells, Human Bone Marrow Macrophages, Primary Keratinocytes, SKOV3, MM-AN, MEF and CD 34+.

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