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MINI26

Sigma-Aldrich

PKH26 Red Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

Synonyme(s) :

Red PKH membrane label kit

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1 KIT
351,00 €

351,00 €

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1 KIT
351,00 €

About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.32

351,00 €

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.

Conditionnement

pkg of 1 kit

Niveau de qualité

200

Fabricant/nom de marque

Distributed for Phanos Technologies

Conditions de stockage

protect from light

Technique(s)

flow cytometry: suitable

Fluorescence

λex 551 nm; λem 567 nm (PKH26 dye)

Application(s)

cell analysis
detection

Méthode de détection

fluorometric

Conditions d'expédition

ambient

Température de stockage

room temp

Description générale

This kit provides enough dye for up to 25 samples of 20 million cells each and enough diluent for up to 5 samples of 20 million cells each, when using a 2 mL final staining volume containing a final concentration of 2 x 10-6 M of PKH26 dye. Users must determine the optimal dye and cell concentrations from their cell type(s) and experimental purposes.

Application

PKH26 red fluorescent cell linker mini kit has been used for:
  • labelling microglial[1], natural killer cells[2] and preadipocytes[3]
  • tracking of viable transplanted adipose-derived stem cells[4]
  • in vitro proliferation studies of bone marrow stem cells[5]
  • general cell membrane labeling, including for in vitro cell labeling and long term in vivo cell tracking.

Liaison

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Informations légales

CellVue is a registered trademark of Phanos Technologies

Composants de kit seuls

Réf. du produit
Description
  • Diluent C 10 mL
  • PKH26 Cell Linker in ethanol .1 mL

Pictogrammes

FlameExclamation mark
GHS02,GHS07

Mention d'avertissement

Danger

Mentions de danger

H225,H319

Classification des risques

Eye Irrit. 2 - Flam. Liq. 2

Code de la classe de stockage

3 - Flammable liquids

Point d'éclair (°F)

57.2 °F - closed cup

Point d'éclair (°C)

14 °C - closed cup

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
MKCV7372
MKCV8652
MKCR6076
MKCQ0938
MKCQ9076

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Human leukocyte antigen-G1 inhibits natural killer cytotoxicity through blocking the activating signal transduction pathway and formation of activating immunologic synapse
Yu Y, et al.
Human immunology, 69(1), 16-23 (2008)
Imaging of peripheral benzodiazepine receptor expression as biomarkers of detrimental versus beneficial glial responses in mouse models of Alzheimer's and other CNS pathologies
Ji B, et al.
The Journal of Neuroscience, 28(47), 12255-12267 (2008)
Adipose tissue regeneration in vivo using micronized acellular allogenic dermis as an injectable scaffold
Lee HY, et al.
Aesthetic Plastic Surgery, 38(5), 1001-1010 (2014)
Transplanted bone marrow stem cells relocate to infarct penumbra and co-express endogenous proliferative and immature neuronal markers in a mouse model of ischemic cerebral stroke
Zhang XM, et al.
BMC Neuroscience, 11(1), 138-138 (2010)
Maria Giovanna Gandolfi et al.
Nanomaterials (Basel, Switzerland), 10(3) (2020-03-04)
Exosomes derived from mesenchymal stem cells are extracellular vesicles released to facilitate cell communication and function. Recently, polylactic acid (PLA), calcium silicates (CaSi), and dicalcium phosphate dihydrate (DCPD) have been used to produce bioresorbable functional mineral-doped porous scaffolds-through thermally induced
Afficher tous les articles scientifiques apparentés

Articles

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

Lipophilic Dye Kits for Cell Tracking

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

Cell Tracking with Lipophilic Membrane Dyes

Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

PKH and CellVue® Claret Fluorescent Cell Linker Dyes

PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.

Questions

  1. Is it possible to lable cells with PKH26 at 4oC?

    1 answer

    1. The membrane dyes will remain stable during the monoclonal staining at 4 deg C; however, capping of the monoclonal antibodies is highly probable if the general cell membrane labeling is carried out at ambient temperature subsequent to antibody labeling. As additional information may be required to address the question completely, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquiries" under the Products Section with all the information needed so that a member of the Technical Service team can reach out to assist further.

      For more information, please refer to the product data-sheet below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/475/mini26bul.pdf.

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