Accéder au contenu
Merck
Toutes les photos(3)

Principaux documents

Voir toute la documentation

R8004

Sigma-Aldrich

Rhodamine 123

Powder

Synonyme(s) :

2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme

Sélectionner une taille de conditionnement

5 MG
58,40 €
25 MG
202,00 €
100 MG
632,00 €

58,40 €

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros
Toutes les photos(3)

Sélectionner une taille de conditionnement

Changer de vue
5 MG
58,40 €
25 MG
202,00 €
100 MG
632,00 €

About This Item

Formule linéaire :
C21H17ClN2O3
Numéro CAS:
62669-70-9
Poids moléculaire :
380.82
Beilstein:
6030951
Numéro CE :
263-687-8
Numéro MDL:
MFCD00012664
Code UNSPSC :
12171500
ID de substance PubChem :
24899403
Nomenclature NACRES :
NA.47

58,40 €

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros

Nom du produit

Rhodamine 123, mitochondrial specific fluorescent dye

Niveau de qualité

100

Forme

powder

Solubilité

ethanol: 1 mg/mL

Application(s)

diagnostic assay manufacturing
hematology
histology

Température de stockage

room temp

Chaîne SMILES 

Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24

InChI

1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H

Clé InChI

MYFATKRONKHHQL-UHFFFAOYSA-N

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Application

Rhodamine 123 is useful as a laser dye and to have selective cell growth effects. It has also been used to measure mitochondrial membrane potential.[1][2]

Actions biochimiques/physiologiques

Rhodamine 123 is a fluorescent dye most commonly used in flow cytometry as functional reporter for P-glycoprotein (Pgp).[3] It is part of the rhodamine family of flurone dyes.[4] The dye is mostly used to assess mitochondrial bioenergetics in living cells. Functional assays for multi-drug resistance (MDR) proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of multi-drug resistance protein-1 (MRP1).[4]
Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
MKCN7507
MKCF0567
MKBX9652V
MKBC2653V
MKBC2653

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Paul E Abraham et al.
The Plant journal : for cell and molecular biology, 103(2), 869-888 (2020-04-22)
Crassulacean acid metabolism (CAM) is a specialized mode of photosynthesis that offers the potential to engineer improved water-use efficiency (WUE) and drought resilience in C3 plants while sustaining productivity in the hotter and drier climates that are predicted for much
Characterization of Rhodamine-123 as a Tracer Dye for Use In In vitro Drug Transport Assays
Samantha
PLoS ONE (2012)
BAG5 Interacts with DJ-1 and Inhibits the NeuroprotectiveEffects of DJ-1 to Combat Mitochondrial Oxidative Damage
Li-xia Qin
Oxidative Medicine and Cellular Longevity (2017)
Role of Mitochondrial Metabolism in the Control of
Early Lineage Progression and Aging Phenotypes in
Adult Hippocampal Neurogenesis
Ruth Beckervordersandforth
Neuron (2016)
S D Bernal et al.
Science (New York, N.Y.), 218(4577), 1117-1119 (1982-12-10)
Rhodamine-123, a cationic laser dye, markedly reduced the clonal growth of carcinoma cells but had little effect on nontumorigenic epithelial cells in vitro. This selective inhibitory effect of Rhodamine-123 on some carcinomas is unusual since known anticancer drugs, such as
Afficher tous les articles scientifiques apparentés

Questions

1–6 of 6 Questions  

  1. Is Rhodamine 123 suitable for mitochondrial staining?

    1 answer

    1. The suitability of Rhodamine 123 for staining mitochondria is not specifically tested during quality control testing. However, it is expected that Rhodamine 123 can be used for this purpose. Conn's Biological Stains, 10th Edition does state that Rhodamine 123 can be used for staining mitochondria. Reference: Celis JS (1998) Cell Biology: A Laboratory Handbook, 2nd Edition v1, Academic Press, San Diego.

      Helpful?

  2. Which one is the better concentration for mitochondrial visualization in immunofluoressence microscopy? I´m using mononuclear cells. Thanks.

    1 answer

    1. Testing R8004 for mitochondrial staining is not part of the standard quality control testing for product R8004. Did find a reference that mentions using Rhodamine 123 at 10 ug/ml for the staining of mitochondria

      Helpful?

  3. What are the excitation and emission wavelengths for Rhodamine 123, (Product Nos. 83702 and R8004)?

    1 answer

    1. Using ethanol as the solvent, the excitation maximum is  511 nm and the emission maximum is 534 nm.  The recommended excitation and emission wavelengths in PBS are 488 and 515-575 nm, respectively.

      Helpful?

  4. What is the solubility of Rhodamine 123, products 83702 and R8004?

    1 answer

    1. Rhodamine 123 is soluble in ethanol at 20 mg/mL, and has been reported soluble in ether, various fats and lipids, and has limited solubility in water (no concentration given).

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. Why would one select Rhodamine 123, products 83702 and R8004, instead of other rhodamine compounds?

    1 answer

    1. Due to is comparatively low toxicity, Rhodamine 123 is the dye of choice for visualizing mitochondria or for cell viability measurements.

      Helpful?

Reviews

No rating value

Active Filters

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique