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PKH26GL

Sigma-Aldrich

Kit de marquage cellulaire à fluorescence rouge au PKH26 pour le marquage des membranes cellulaires à usage général

Distributed for Phanos Technologies

Synonyme(s) :

Red PKH membrane dye

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1 KIT
1 670,00 €

1 670,00 €

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Changer de vue
1 KIT
1 670,00 €

About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.32

1 670,00 €

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.

Conditionnement

pkg of 1 kit

Niveau de qualité

200

Fabricant/nom de marque

Distributed for Phanos Technologies

Conditions de stockage

protect from light

Fluorescence

λex 551 nm; λem 567 nm (PKH26 dye)

Méthode de détection

fluorometric

Conditions d'expédition

ambient

Température de stockage

room temp

Catégories apparentées

Description générale

PKH26 est un colorant fluorescent rouge. Ce colorant des membranes lipophiles est largement utilisé pour déterminer la prolifération cellulaire de cellules hétérogènes par cytométrie en flux.[1]

Application

Ce kit est destiné au marquage des membranes cellulaires à usage général. Il a été caractérisé dans un certain nombre de systèmes modèles et s′est avéré utile pour le marquage cellulaire in vitro, les études de prolifération in vitro et le traçage cellulaire in vivo à long terme. La demi‑vie en élution de PKH26 dans des globules rouges marqués de lapin est supérieure à 100 jours. Cette stabilité accrue trouve son intérêt dans les études in vivo à long terme.
Le kit de marquage cellulaire en fluorescence rouge PKH26 est destiné au marquage universel des membranes cellulaires. Il a été caractérisé dans un certain nombre de modèles et a démontré son utilité pour le marquage de cellules in vitro, l'étude de la prolifération cellulaire in vitro et le suivi cellulaire à long terme in vivo.[2][3] Sa demi-vie après élution des globules rouges de lapin marqués est supérieure à 100 jours. Cette grande stabilité s'avère utile pour les études in vivo à long terme.

Liaison

Pour obtenir des détails techniques sur les colorants fluorescents PKH et CellVue® destinés au marquage cellulaire, notamment une bibliographie complète, rendez-vous ici.

Informations légales

CellVue is a registered trademark of Phanos Technologies

Composants de kit seuls

Réf. du produit
Description
  • Diluent C 6 x 10
  • PKH26 Cell Linker in ethanol .5 mL

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

FlameExclamation mark
GHS02,GHS07

Mention d'avertissement

Danger

Mentions de danger

H225,H319

Classification des risques

Eye Irrit. 2 - Flam. Liq. 2

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

57.2 °F - closed cup

Point d'éclair (°C)

14.0 °C - closed cup

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
MKCV7397
MKCV4102
MKCV7789
MKCR6075
MKCQ6477

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Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

EphA3 as a target for antibody immunotherapy in acute lymphoblastic leukemia
Charmsaz S, et al.
Leukemia, 31(8), 1779-1779 (2017)
Ofra Ben Menachem-Zidon et al.
PloS one, 14(6), e0218081-e0218081 (2019-06-14)
The beneficial effect of mesenchymal stem cells (MSCs) on wound healing is mostly attributed to a trophic effect that promotes angiogenesis. Whether MSCs can contribute to the formation of new blood vessels by direct differentiation is still controversial. Pelvic floor
Rafi Chapanian et al.
Biomaterials, 33(10), 3047-3057 (2012-01-21)
The in vivo circulation of hyperbranched polyglycerol (HPG) grafted red blood cells (RBCs) was investigated in mice. The number of HPG molecules grafted per RBC was measured using tritium labeled HPGs ((3)H-HPG) of different molecular weights; the values ranged from
Anne Julie Frenette et al.
Journal of neurotrauma, 29(1), 1-18 (2011-08-19)
In the intensive care unit, dopamine agonists (DA) have been used in traumatic brain injury (TBI) patients to augment or accelerate cognitive recovery and rehabilitation. However, the efficacy and safety of DA in this population is not well established. We
Tracking antigen-driven responses by flow cytometry: Monitoring proliferation by dye dilution
Paul KW, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 73(11), 1019-1034 (2008)
Afficher tous les articles scientifiques apparentés

Articles

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

Lipophilic Dye Kits for Cell Tracking

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

Cell Tracking with Lipophilic Membrane Dyes

Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

PKH and CellVue® Claret Fluorescent Cell Linker Dyes

PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.

Questions

1–10 of 10 Questions  

  1. If we want to use the PKH to label the membrane of fibroblast, and then co-culture them with tumor cells in order to interact, ¿ Will PKH transfer from fibroblast to tumor cells?

    1 answer

    1. PKH dyes are routinely used in co-culture situations. Because staining is nearly instantaneous, rapid and homogeneous dispersion of cells in dye solution is essential. There should be no excess or unincorporated dye in the medium prior to mixing or co-culturing the cell lines. Free dye will be taken up by all cells. See the links below to the product protocol as well as two additional co-culture studies using PKH dyes:

      Protocol
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/475/mini26bul.pdf

      Co-culture in scaffolds
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155425/

      Co-culture, mixed monolayer, and on Transwell
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5168417/

      Helpful?

  2. Are cells lost during the PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling staining process?

    1 answer

    1. Over-labeling of the cells will result in loss of membrane integrity and reduced cell recovery.  Methods for improving cell viability can be found on the troubleshooting guide.

      Helpful?

  3. When using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling, how long will the cells remain stained after treatment?

    1 answer

    1. Labeled cells that have been washed can be visualized in culture up to 100 days after staining (for non-dividing cells). The dye itself is stable and will divide equally when the cells divide. After staining with PKH dyes, you can observe as many as 8 divisions depending on how brightly the cells were stained initially and the amount of surface area on the cells. Most commonly, 4-6 divisions can be visualized.

      Helpful?

  4. What method of fixation can be used for tissue/cells with the PKH Fluorescent Cell Linker Kit for General Cell Membrane Labeling dyes?

    1 answer

    1. A protocol for visualization of tissue sections can be found in the technical bulletin.  For visualization of stained cells by immunofluorescence or flow cytometry, the cells can be fixed in 2% paraformaldehyde for 15 minutes. The use of other organic solvents will extract the dye from the cells.  If internal labeling is desired, the cells can be permeabilized with saponin (50-75 μg/mL). Here is the link to the Troubleshooting Guide.

      Helpful?

  5. Will Product PKH26GL, PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling Products, stain dead cells?

    1 answer

    1. As long as the cell has an intact membrane, the PKH26 dye can label the cell.

      Helpful?

  6. What is the difference between PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling Products PKH26GL and PKH26PCL?

    1 answer

    1. The general labeling kit (Product No. PKH26GL) will label the membrane of the cells.  For phagocytic cells, Product No. PKH26PCL is used.  This kit utilizes dye aggregates for specific labeling of phagocytic cells in the presence of other cell types.

      Helpful?

  7. Does the dye leak from the cells after labeling when using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling?

    1 answer

    1. In general, slow loss of fluorescence has been observed with PKH1 and PKH2 in in vivo studies and it is likely that PKH67 may also slowly lose fluorescence. Leakage (or loss of staining intensity) may also occur due to cell-to-cell transfer of the dye. The most common cause of cell-to-cell dye transfer is inadequate washing of the cells. Wash cells 3-5 times after labeling and transfer samples to new tubes between washes.

      Helpful?

  8. Product PKH26GL, PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, is a red dye.  Do you offer a kit that stains the cells green?

    1 answer

    1. We offer Product numbers:PKH2GL Both of these kits stain the cells with a dye that emits in the green fluorescent range.

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  10. How many cells can be stained with PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, Products PKH26GL and MINI26?

    1 answer

    1. For Product PKH26GL, when using the concentrations indicated in the product bulletin, you can stain 30 samples (1x107 cells/sample).The kit provides 500 ul of PKH26 dye solution.  You use 4 ul per sample.  There is enough dye in the kit to stain 125 samples.The kit provides 60 mls of Diluent C.  You use 2 mls per sample (1 ml for dilution of the dye, 1 ml for dilution of the cells).  There is enough diluent for 30 samples.You can purchase additional diluent as Product CGLDILIf you need less dye, you can purchase Product MINI26 which contains 100 ul of dye and 10 mls of Diluent C.  This kit contains enough dye to stain 25 samples and enough diluent to stain 5 samples.

      Helpful?

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