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P2714

Sigma-Aldrich

Protease Inhibitor Cocktail

lyophilized powder, for the inhibition of serine, cysteine and metallo-proteases, for general use, lyophilized powder

Synonyme(s) :

Protease Inhibitor Cocktail from bovine lung, Protease Inhibitor Mix, Protease inhibitor

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77

product name

Protease Inhibitor Cocktail powder, for general use, lyophilized powder

Source biologique

bovine lung

Niveau de qualité

300

Forme

lyophilized powder

Température de stockage

−20°C

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Description générale

The protease inhibitor cocktail has been optimized and tested for general use. It consists of water-soluble protease inhibitors that effectively inhibit serine, cysteine, and metalloproteases. This product is supplied as a lyophilized powder.

Spécificité

Inhibits serine, cysteine, and metallo-proteases.

Application

Protease Inhibitor Cocktail powder has been used as a component:
  • in radioimmunoprecipitation assay buffer to lyse the avian crystalline lens samples for western blot analysis
  • in phosphate buffer solution (PBS) for grinding recombinant protein expressed leaves to purify virus-like particles (VLPs)
  • in PBS to dissolve lyophilized skin secretion sample of A. loloensis for peptide purification
  • in 0.1 M NaCl solution to wash and collect skin secretions of frog

Actions biochimiques/physiologiques

The components of this mixture, such as AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.

Composants

This mixture contains individual components, including:

AEBSF at 2 mM
Aprotinin at 0.3 μM
Bestatin at 116 μ
E-64 at 14 μM
EDTA at 1 mM
Leupeptin at 1 μM

Each component has specific inhibitory properties.

Attention

The lyophilized powder is stable for 4 years when stored at -20°C.

Quantité

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Notes préparatoires

This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.

Autres remarques

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Produit(s) apparenté(s)

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Description
Tarif

P8465

Protease Inhibitor Cocktail powder, for use with bacterial cell extracts, lyophilized powder

P8340

Cocktail d'inhibiteurs de protéases, for use with mammalian cell and tissue extracts, DMSO solution

P8215

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P8849

Cocktail d'inhibiteurs de protéases, for use in purification of Histidine-tagged proteins, DMSO solution

P9599

Cocktail d'inhibiteurs de protéases, for plant cell and tissue extracts, DMSO solution

P1860

Cocktail d'inhibiteurs de protéases, for use in tissue culture media, DMSO solution

S8820

Comprimés d'inhibiteurs de protéases SIGMAFAST™, For General Use

S8830

Cocktail d′inhibiteurs de protéases SIGMAFAST™, comprimés, sans EDTA, for use in purification of Histidine-tagged proteins

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H315 - H319

Classification des risques

Eye Irrit. 2 - Skin Irrit. 2

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Five novel antimicrobial peptides from skin secretions of the frog, Amolops loloensis
Wang M, et al.
Journal of biological and chemical chronicles, 155(1), 72-76 (2010)
Safety and immunogenicity of plant-produced African horse sickness virus-like particles in horses
Dennis SJ, et al.
Veterninary Research, 49(1), 1-6 (2018)
F Maier et al.
Osteoarthritis and cartilage, 27(9), 1382-1391 (2019-05-24)
Given the structural changes associated with the progression of Osteoarthritis (OA), we hypothesized that patterns of through-thickness, large-strain shear evolve with early-stage OA. We therefore aimed to determine whether and how patterns of shear strains change during early-stage OA to
Giorgio Ramadori et al.
Nature communications, 10(1), 3545-3545 (2019-08-09)
Tens of millions suffer from insulin deficiency (ID); a defect leading to severe metabolic imbalance and death. The only means for management of ID is insulin therapy; yet, this approach is sub-optimal and causes life-threatening hypoglycemia. Hence, ID represents a
Céline Méhats et al.
PloS one, 3(10), e3445-e3445 (2008-10-23)
Prolonged neonatal exposure to hyperoxia is associated with high mortality, leukocyte influx in airspaces, and impaired alveolarization. Inhibitors of type 4 phosphodiesterases are potent anti-inflammatory drugs now proposed for lung disorders. The current study was undertaken to determine the effects
Afficher tous les articles scientifiques apparentés

Articles

Aprotinin

While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.

Contenu apparenté

Protease Inhibitor Selection: Avoid Protein Loss in Sample Prep

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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