N6009
Nerve Growth Factor-2.5S from murine submaxillary gland
NGF-2.5S, lyophilized powder, suitable for cell culture
Synonyme(s) :
NGF-2.5S
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About This Item
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme
lyophilized powder
Puissance
0.1-30 ng/mL EC50 (using PC-12 cells)
Poids mol.
26.5 kDa
Conditionnement
pkg of 4X25 μg
Conditions de stockage
avoid repeated freeze/thaw cycles
Technique(s)
cell culture | mammalian: suitable
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Informations sur le gène
mouse ... Ngf(18049)
Catégories apparentées
Description générale
Nerve growth factor (NGF) is produced by different cell types: structural, accessory, and immune cells. It belongs to the neurotrophin family of trophic factors.
Application
Nerve Growth Factor-2.5S from murine submaxillary gland has been used:
- in Ham′s F-12 medium for performing growth cone collapse assay using dorsal root ganglions (DRGs)
- as a media supplement in the “process compartment” for microfluidic chamber studies
- to treat mid-luteal cells to study its effect on choline acetyltransferase (CHAT) expression
Actions biochimiques/physiologiques
Nerve growth factor (NGF) is neurotrophic and maintains sympathetic and sensory neurons of the peripheral nervous system and forebrain cholinergic neurons. NGF promotes the development of neurite-like filaments from chick embryo dorsal root ganglia and rat PC12 pheochromocytoma cells. NGF may have a role in in vivo fetal development and nerve regeneration especially, after ischemic, surgical, or chemical injuries. NGF may also be associated with the central nervous system. It exhibits ameliorating effects against ulcers (human cutaneous, corneal ulcers, and pressure ulcers), glaucoma, maculopathy, and retinitis pigmentosa. NGF-2.5S is a type of NGF-b originally derived under dissociative conditions and often varies from NGF-b due to proteolysis arising during its purification.
Autres remarques
2.5S subunit of nerve growth factor-7S (NGF-7S) is essentially the β-subunit when isolated from male mouse submaxillary glands under initially dissociative conditions by a modification of the method of Bocchini and Angeletti.
Forme physique
Lyophilized from a 0.2 μm filtered solution in sodium acetate buffer.
Remarque sur l'analyse
The biological activity is measured using a 3-day MTT assay.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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