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MTOX1002

Sigma-Aldrich

BCRP Knockout Caco-2 Cells

Human male colorectal tissue, adenocarcinoma

Synonyme(s) :

C2BBe1 Cells BCRP (-/-/-/-)

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About This Item

Code UNSPSC :
41106514
Nomenclature NACRES :
NA.81

product name

BCRP Knockout Caco-2 Cells, one vial

Source biologique

human male colorectal tissue (Source disease: adenocarcinoma)

Forme

liquid

Technique(s)

drug transporter assay: suitable
permeability assay: suitable

Numéro d'accès OMIM

603756

Application(s)

ADME/TOX

Température de stockage

−196°C

Informations sur le gène

human ... ABCG2(9429)

Description générale

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The BCRP knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCG2 (BCRP) efflux transporter.

Application

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

Caractéristiques et avantages

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:
  • A functional knockout of the BCRP gene eliminates the reliance on chemical inhibitors to determine if a compound is an BCRP substrate
  • The vial format enables the BCRP knockout cells to be included in standard drug transporter protocols
  • Human assay with no interference from animal inhibitors
  • Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

Informations légales

ADME/Tox Cell Lines License

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d'importation et d'exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L'acheteur (c'est-à-dire l'utilisateur final) est tenu d'obtenir une autorisation d'importation auprès du Ministère français de la Recherche, mentionné à l'article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l'autorisation d'importation requise.

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Réf. du produit
Description
FDS
  • MTOX1002BCRP Knockout Caco-2 Cells, one vial 1 vialFDS

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

P Artursson
Journal of pharmaceutical sciences, 79(6), 476-482 (1990-06-01)
A human intestinal cell line, Caco-2, was used as a model to study the passive diffusion of drugs across intestinal epithelium. The cells formed continuous monolayers when grown on permeable filters of polycarbonate. After 10 days in culture, the monolayers
V Pade et al.
Journal of pharmaceutical sciences, 87(12), 1604-1607 (1999-04-03)
The objective of this investigation was to establish a relationship between drug permeability and solubility in vitro and the extent of drug absorption in humans. We selected drugs with varying permeabilities and solubilities with the aim of establishing a relationship
X Wu et al.
Pharmaceutical research, 17(2), 209-215 (2000-04-06)
The purpose of this study was to elucidate the mechanisms by which an HMG-CoA reductase inhibitor, atorvastatin (an organic acid with a pKa of 4.46), was transported in the secretory and absorptive directions across Caco-2 cell monolayers. Caco-2 cells were
Kathleen E Sampson et al.
Drug metabolism and disposition: the biological fate of chemicals, 43(2), 199-207 (2014-11-13)
Membrane transporters P-glycoprotein [P-gp; multidrug resistance 1 (MDR1)], multidrug resistance-associated protein (MRP) 2, and breast cancer resistance protein (BCRP) affect drug absorption and disposition and can also mediate drug-drug interactions leading to safety/toxicity concerns in the clinic. Challenges arise with
M J Briske-Anderson et al.
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.), 214(3), 248-257 (1997-03-01)
The Caco-2 cell line is used by many investigators as a model of the intestinal epithelium to study nutrient uptake and transport. Our goal was to create an awareness of inherent variabilities in the Caco-2 cell line which may influence
Afficher tous les articles scientifiques apparentés

Articles

The Role of Intestinal Efflux Transporters In Drug Absorption

We presents an article on The Role of Intestinal Efflux Transporters In Drug Absorption.

Drug transport assays in a 96-well system: reproducibility and correlation to human absorption

Application note on Drug transport assays in a 96-well system using Millicell-96 System from Millipore.

Caco-2 Intestinal Drug Transporter Models for More Predictive Drug Absorption

Utilize these Caco-2 cell based assay tools for screening small molecule drug compounds prior to clinical studies and submission to regulatory agencies.

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