The Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) has not been tested with bacteria samples, so there are no specific recommendations available for this application. Additionally, this kit has not been tested with mammalian lysates. The lysis buffer required for the assay may be incompatible with the buffers provided, and the substrate may not be compatible or may cross-react with other biological components found in a lysate.
MAK404
Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric)
sufficient for 100 colorimetric tests
Synonyme(s) :
CA Enzyme Activity Assay Kit (Colorimetric)
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About This Item
Code UNSPSC :
12161503
Produits recommandés
Méthode de détection
colorimetric
Maladie(s) pertinente(s)
endocrinological disorders, diabetes; cancer; hematological disorder
Température de stockage
−20°C
Description générale
Carbonic anhydrases (CA) are zinc enzymes present in both prokaryotes and eukaryotes. They catalyze efficiently the reversible hydration of CO2 to bicarbonate. Their important patho-physiological roles in respiration, pH and CO2 homeostasis, secretion, gluconeogenesis, ureagenesis etc. make it an important drug target. Characteristic increase and decrease of CA Activity is observed in different physiological conditions and diseases such as anemia, Thalassemia, Hypothyroidism, Hyperthyroidism, and in several cases of lung and liver diseases and Leukemia.
Caractéristiques et avantages
Carbonic Anhydrase (CA) Activity Assay Kit:
- Compatible with high-throughput handling systems.
Adéquation
The kit is suitable for the detection of carbonic anhydrase activity in hemolysates and serum, as well as the determination of enzymatic activity of purified CA.
Principe
The assay utilizes the esterase activity of an active CA on an ester substrate which releases a chromophore.The released product can be easily quantified using a spectrophotometric multi-well plate reader. In the presence of a CA specific inhibitor, the enzyme loses its activity which results in a decrease of absorbance.To measure specific CA activity, the CA inhibitor Acetazolamide is included.Acetazolamide is a potent inhibitor of CA I, CA II, CA IV, CA IX, CA XII etc. except CA III.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 3
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Is MAK404-1KT Carbonic Anhydrase Activity Assay Kit (Colorimetric) suitable for use with bacteria and mammalian tissue lysates?
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