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C2624

Sigma-Aldrich

Anhydrase carbonique from bovine erythrocytes

≥95% (SDS-PAGE), specific activity ≥3,500 W-A units/mg protein, lyophilized powder

Synonyme(s) :

Déshydratase carbonique, Hydrolase de carbonate

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100 MG
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500 MG
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About This Item

Numéro CAS:
9001-03-0
Numéro de classification (Commission des enzymes):
4.2.1.1 (BRENDA, IUBMB)
Numéro CE :
232-576-6
Numéro MDL:
MFCD00081467
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

280,00 €

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Source biologique

bovine erythrocytes

Essai

≥75% protein basis (biuret)
≥95% (SDS-PAGE)

Forme

lyophilized powder

Activité spécifique

≥3,500 W-A units/mg protein

Solubilité

deionized water: >10 mg/mL

Température de stockage

2-8°C

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Application

Carbonic anhydrase, from bovine erythrocytes, is used to create carbon dioxide capture systems and to research various purification techniques [1]. Carbonic anhydrase is also used to study acid-base regulation in fish and carbonic anhydrase type II deficiency syndrome [2][3].

Actions biochimiques/physiologiques

Carbonic Anhydrase is a zinc-containing enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate. One of its main physiological roles is to maintain the acid-base balance in blood and other tissues. Lack of carbonic anhydrase results in carbonic anhydrase type II deficiency syndrome, which is an autosomal recessive disease that causes osteopetrosis, renal tubular acidosis and brain calcifications [3].

Définition de l'unité

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Certificats d'analyse (COA)

Lot/Batch Number
0000351730
0000351729
0000302263
0000302262
0000351729

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Consulter la Bibliothèque de documents

Nicolas Ghéczy et al.
RSC advances, 10(32), 18655-18676 (2020-05-18)
Enzyme-catalysed cascade reactions in flow-through systems with immobilised enzymes currently are of great interest for exploring their potential for biosynthetic and bioanalytical applications. Basic studies in this field often aim at understanding the stability of the immobilised enzymes and their
Abdur Rauf et al.
Chemistry & biodiversity, 14(6) (2017-02-17)
Plant phenolics are known to display many pharmacological activities. In the current study, eight phenolic compounds, e.g., luteolin 5-O-β-glucoside (1), methyl rosmarinate (2), apigenin (3), vicenin 2 (4), lithospermic acid (5), soyasaponin II (6), rubiadin 3-O-β-primeveroside (7), and 4-(β-d-glucopyranosyloxy)benzyl 3,4-dihydroxybenzoate
Joana da Costa Ores et al.
Chemosphere, 88(2), 255-259 (2012-04-20)
This work presents a study of industrially applicable techniques to obtain a biologically supported carbon dioxide capture system, based on the extraction of carbonic anhydrase from bovine blood. Carbonic anhydrase is a metalloenzyme which catalyzes the reversible hydration of carbon
Marco Catalano et al.
Analytical chemistry, 92(15), 10822-10829 (2020-07-04)
The availability of reliable methods for the characterization of the binding of small molecule ligands to protein targets is crucially important for drug discovery. We have adapted a method, routinely used for the characterization of monoclonal antibodies (enzyme-linked immunosorbent assay
K M Gilmour et al.
The Journal of experimental biology, 212(Pt 11), 1647-1661 (2009-05-19)
Carbonic anhydrase (CA) is the zinc metalloenzyme that catalyses the reversible reactions of CO(2) with water. CA plays a crucial role in systemic acid-base regulation in fish by providing acid-base equivalents for exchange with the environment. Unlike air-breathing vertebrates, which
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Questions

1–4 of 4 Questions  

  1. What is recommand usage temperature and pH range?

    1 answer

    1. The activity of this enzyme is established using a 0.02 M Tris buffer with a starting pH of 8.3 at 0°C. One unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. However, the optimum pH and temperature is highly dependent on the specific application. There are a number of publications regarding the working parameters for this material. Please see the link below to review an example:
      https://pubmed.ncbi.nlm.nih.gov/10701452/#:~:text=It%20was%20determined%20that%20the,61706%20Dalton%20by%20gel%20filtration.

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  2. What is the recommended storage conditions for "Carbonic Anhydrase, from bovine erythrocytes?

    1 answer

    1. The recommended storage conditions for Solution of Carbonic Anhydrase, from bovine erythrocytes may be frozen at –20 °C for future use. It is advised to avoid repeated freezing and thawing. Stock solutions may be dispensed into working aliquots, frozen, and then discarded after 2–3 uses.

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  3. What is the isozyme I or isozyme II of Carbonic Anhydrase from bovine erythrocytes (C2624 Carbonic Anhydrase)?

    1 answer

    1. The product C2624, Carbonic Anhydrase from bovine erythrocytes, consists mainly of CA II. However, there are also small levels of CA I and CA IV present.

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  4. Is it possible to obtain information about the type of Carbonic Anhydrase (I or II) included in item C2624-carbonic anhydrase?

    1 answer

    1. Product C2624, Carbonic Anhydrase from bovine erythrocytes, with a purity of ≥95% (SDS-PAGE), consists predominantly of CA II, with small levels of CA I and CA IV present.

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