The principle of this kit is the reaction of formaldehyde with acetoacetanilide in the presence of ammonia. Neither a MESNA (4-Morpholineethanesulfonic acid, sodium) buffer nor EDTA in the test sample would be expected to interfere with this reaction. Having said that, it would be appropriate to run a blank containing MESNA and EDTA, and standards spiked with MESNA and EDTA, to confirm they they are not interfering with the test result...that is, making it either artificially high or low.
MAK131
Formaldehyde Assay
sufficient for 100 fluorometric tests
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
412,00 €
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Devis pour commande en gros
Produits recommandés
Utilisation
sufficient for 100 fluorometric tests
Méthode de détection
fluorometric
Maladie(s) pertinente(s)
cancer
Température de stockage
2-8°C
Catégories apparentées
Description générale
Formaldehyde is widely employed in industry for a wide range of applications. It acts as a disinfectant and biocide, and is utilized as a tissue fixative and embalming agent.[1] Formaldehyde is a key metabolite produced during normal metabolism and contributes to methylation reactions and protein and nucleic acid synthesis. Increased production of formaldehyde has been observed in many cancer cell lines and increased formaldehyde concentration in urine has been associated with bladder and prostrate cancer.[2]
Application
- Modified Natural Rubber as a Simple Chemical Sensor with Smartphone Detection for Formaldehyde Content in a Seafood Sample.: This article explores the use of modified natural rubber as a chemical sensor, paired with smartphone detection, to measure formaldehyde content in seafood. This approach highlights the potential for accessible and mobile detection systems in food safety applications (Yeerum et al., 2022).
- Optical enzymatic formaldehyde biosensor based on alcohol oxidase and pH-sensitive methacrylic-acrylic optode membrane.: The study develops an optical enzymatic biosensor for formaldehyde, using alcohol oxidase with a pH-sensitive membrane, offering a novel approach for quick and sensitive detection in various analytical applications (Nurlely et al., 2022).
Caractéristiques et avantages
Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.
Adéquation
Suitable for the detection of formaldehyde in urine and other biological samples.
Principe
The Formaldehyde Assay kit provides a simple and direct procedure for measuring formaldehyde levels in urine and other biological samples. In this assay, formaldehyde is derivatized with acetoacetanilide in the presence of ammonia resulting in a fluorescent (λex = 370/λem = 470 nm) product, proportional to the formaldehyde present.
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 1 - STOT SE 3
Organes cibles
Eyes,Central nervous system, Respiratory system
Code de la classe de stockage
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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Les clients ont également consulté
Cristina A Nadalutti et al.
Scientific reports, 10(1), 5575-5575 (2020-03-30)
Formaldehyde (FA) is a simple biological aldehyde that is produced inside cells by several processes such as demethylation of DNA and proteins, amino acid metabolism, lipid peroxidation and one carbon metabolism (1-C). Although accumulation of excess FA in cells is
Lysine-specific demethylase 1 in breast cancer cells contributes to the production of endogenous formaldehyde in the metastatic bone cancer pain model of rats.
Liu J, et al.
PLoS ONE, 8(3), e58957-e58957 (2013)
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Traditional diosgenin manufacturing process has led to serious environmental contamination and wastewater. Clean processes are needed that can alternate the diosgenin production. The β-glucosidase FBG1, cloned from Fusarium sp. CPCC 400709, can biotransform trillin and produce diosgenin. In this study
Formaldehyde in the workplace and in the home: exploring its clinical toxicology.
Montanaro A.
Laboratory Medicine, 27(11), 752-758 (2015)
Joan Artigas et al.
Environmental pollution (Barking, Essex : 1987), 259, 113951-113951 (2020-01-16)
Environmental dissolved organic matter (DOM) has been proved to increase microbial population sizes and stimulate the degradation of some pesticide molecules. Among these molecules, the present study investigated the biodegradation of the herbicide glyphosate depending on photoautotrophs DOM supply in
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Is the assay compatible with MES buffer? Is EDTA affecting the reaction?
1 answer-
Helpful?
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What is the limit of detection that the MAK131 Formaldehyde Assay is capable of?
1 answer-
The kit is capable of detecting formaldehyde down to 1.5 micromolar or 45 ppb. The upper end of detection for undiluted samples is 100 micromolar, which is the highest concentration of the standard curve.
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