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MAK126

Sigma-Aldrich

Bilirubin Assay Kit

sufficient for 180 colorimetric tests

Synonyme(s) :

Hematoidin Assay Kit

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1 KIT
395,00 €

395,00 €

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1 KIT
395,00 €

About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

395,00 €

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros

Utilisation

sufficient for 180 colorimetric tests

Méthode de détection

colorimetric

Maladie(s) pertinente(s)

hematological disorder; gastrointestinal diseases

Température de stockage

2-8°C

Catégories apparentées

Description générale

Bilirubin, also known as hematoidin, is a degradation product formed as a result of heme catabolism in the liver. Bilirubin circulates in the blood stream as either the unconjugated insoluble form (indirect bilirubin) or the soluble glucuronide-conjugated form (direct bilirubin). Conjugated bilirubin moves from the bile canaliculi of the liver to the gall bladder where it is excreted into the small intestine during digestion. High levels of bilirubin can result in jaundice and may indicate liver disease, blood disorders, or blockage of the bile ducts.

Application

Bilirubin Assay Kit has been used to measure the total bilirubin concentration in samples.[1][2]

Caractéristiques et avantages

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Adéquation

Suitable for the detection of of both total and conjugated bilirubin in serum and plasma samples.

Principe

This assay, based on the Jendrassik-Grof method, utilizes the reaction of bilirubin with diazotized sulfanilic acid resulting in a colorimetric product measured at 530 nm, proportionate to the bilirubin present in the sample. This assay kit measures both total and conjugated bilirubin.

Code de la classe de stockage

12 - Non Combustible Liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
126CE12A23
126CE12A23
126CE07A16
126CE05A09
126CE05A10

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Ji-Yoon Kim et al.
BMB reports, 53(3), 148-153 (2019-08-14)
Erythropoietin and iron have individually shown beneficial effects on early-phase liver regeneration following partial hepatectomy (PHx); however, there are limited data on the combined effect on late-phase liver regeneration after PHx. Here we examined combined effects of recombinant human erythropoietin
Morgane M Thibaut et al.
Journal of cachexia, sarcopenia and muscle, 12(1), 70-90 (2020-12-23)
Cancer cachexia is a debilitating metabolic syndrome contributing to cancer death. Organs other than the muscle may contribute to the pathogenesis of cancer cachexia. This work explores new mechanisms underlying hepatic alterations in cancer cachexia. We used transcriptomics to reveal
Zhi-Ming Ding et al.
Frontiers in pharmacology, 9, 410-410 (2018-05-17)
Understanding of the temporal changes of hepatic lesions in the progression and regression of non-alcoholic steatohepatitis (NASH) is vital to elucidation of the pathogenesis of NASH, and critical to the development of a strategy for NASH pharmacotherapy. There are challenges
Kimberly J Jasmer et al.
Pigment cell & melanoma research, 33(6), 850-868 (2020-06-20)
Biosynthesis and degradation of heme, an iron-bound protoporphyrin molecule utilized by a wide variety of metabolic processes, are tightly regulated. Two closely related enzymes, heme oxygenase 1 (HMOX1) and heme oxygenase 2 (HMOX2), degrade free heme to produce carbon monoxide
Bin Dong et al.
Journal of lipid research, 58(2), 350-363 (2016-12-13)
The farnesoid X receptor (FXR) plays critical roles in plasma cholesterol metabolism, in particular HDL-cholesterol (HDL-C) homeostasis. Obeticholic acid (OCA) is a FXR agonist being developed for treating various chronic liver diseases. Previous studies reported inconsistent effects of OCA on
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Questions

1–7 of 7 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Can you please confirm me if these assays works with mouse samples?

    1 answer

    1. All three kits have been used with mouse samples.

      Helpful?

  4. What is a typical absorbance range for the 5 mg/dL standard in the MAK126 Bilirubin Assay Kit?

    1 answer

    1. The OD that represents 5mg/dL bilirubin was determined to be 0.52-0.56 during the development of the kit.

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  5. Does this kit work with urine samples?

    1 answer

    1. Kit MAK126 has not been tested on urine samples, so it is not something that can be guaranteed, but it is likely that urine would be okay as long as the concentration falls within the detection range of the kit. Bilirubin does not typically contain urine, so it may not exhibit a response from the kit unless the urine in question comes from a subject with a certain liver condition.

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  6. Can I use the Bilirubin Assay Kit (MK126) to do Bilirubin Assay for mouse?

    1 answer

    1. This Billirubin Assay is not species specific. It has not been tested specifically on mouse serum, however there is no reason to suspect that this kit would not be suitable. Please see the links below to review publications utilizing this product with mouse serum:
      https://doi.org/10.3389/fphar.2018.00410
      https://doi.org/10.1152/ajpheart.00882.2014
      https://doi.org/10.1002/path.5685

      Helpful?

  7. Hola. Deseo medir bilirrubina total en una colección de sueros que se han mantenido congelados a -70ºC varios meses. Necesitaría saber si su kit es recomendable para estas muestras y la cantidad mínima de suero (humano) que se puede usar por reacción. Gcs

    1 answer

    1. Measurement of Total and Direct Bilirubin requires a total of 150 microliters of sample (50 microliters each for Total, Direct, and Sample Blank). Serum (frozen or fresh) is a suitable sample for use with the kit.

      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/254/564/mak126bul.pdf

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