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M9943

Sigma-Aldrich

10X MTP™ Taq Buffer

10X buffer, free of DNA contaminants; use with MTP Taq DNA Polymerase

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About This Item

Code UNSPSC :
41106306
Nomenclature NACRES :
NA.52

Niveau de qualité

200

Forme

liquid

Couleur

colorless

Conditions d'expédition

wet ice

Température de stockage

−20°C

Catégories apparentées

Description générale

DNA contaminants can be introduced into PCR through a number of reagents. To minimize the risk of contaminant DNA during PCR, we offer 10x MTP Taq buffer to be used with MTP Taq DNA Polymerase (Product No. D7442). Each lot of MTP Taq buffer undergoes strict quality control testing to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA polymerase.

Application

10X MTP™Taq Buffer has been used:

  • for the amplification of bacterial 16s rRNA genes from purified DNA
  • to amplify archaeal 16s rRNA
  • along with MTP Taq DNA polymerase (D7442) to amplify specific bacterial 16s rRNA genes from purified DNA

Informations légales

MTP is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Florie Maillard et al.
PloS one, 14(4), e0214660-e0214660 (2019-04-10)
Increased visceral adipose tissue and dysbiosis in the overweight and obese promote chronic inflammation. The aim of this study was to compare the effects of moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT) on the gut-adipose tissue cross-talk in
Camille Mazière et al.
Microbiological research, 252, 126854-126854 (2021-08-29)
In order to understand the effect of human practices on microbial mats organisation, the study aimed to investigate the biodiversity within microbial mats from exploited and abandoned salterns. Despite several attempts, archaeal 16S rRNA gene fragment sequences were not obtained
C E Corless et al.
Journal of clinical microbiology, 38(5), 1747-1752 (2000-05-02)
A set of universal oligonucleotide primers specific for the conserved regions of the eubacterial 16S rRNA gene was designed for use with the real-time PCR Applied Biosystems 7700 (TaqMan) system. During the development of this PCR, problems were noted with
Risk assessment models and contamination management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriology.
B Cherie Millar et al.
Journal of clinical microbiology, 40(5), 1575-1580 (2002-05-01)
Florie Maillard et al.
Cells, 8(1) (2019-01-13)
Crohn's disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics
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Protocoles

Method for PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase (D7442)

MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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