H0273

Monoclonal Anti-Heat Shock Protein 25 antibody produced in mouse

clone IAP-28, ascites fluid

• Numéro MDL: MFCD02189139
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: IAP-28, monoclonal
• Poids mol.: antigen 25 kDa
• Contient: 15 mM sodium azide
• Espèces réactives: chicken, bovine, frog, rabbit, guinea pig, turkey, Drosophila, mouse, rat, human
• Technique(s): immunohistochemistry: suitable using frozen sections of chicken gizzard smooth muscle; indirect ELISA: suitable; indirect immunofluorescence: suitable using formalin-fixed, heat-shocked cultured chick embryo fibroblasts; radioimmunoassay: suitable; western blot: 1:500 using turkey gizzard extract
• Isotype: IgG1
• Numéro d'accès UniProt: P04792
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... HSPB1(3315) ; mouse ... Hspb1(15507) ; rat ... Hspb1(24471)

Description

Description générale

Monoclonal anti-Heat Shock Protein 25 (mouse IgG1) isotype) is derived from the IAP-28 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle. Heat shock (stress) proteins (HSPs) are a group of polypeptides synthesized in response to a wide variety of environmental factors, including biotic and abiotic stressors. These proteins are produced by prokaryotic and eukaryotic cells, and HSPs are among the most conserved molecules in phylogeny. Heat shock protein 25 is encoded by the gene mapped to human chromosome 7q11.23. The encoded protein belongs to the class of small HSPs. Small HSPs aggregate to form characteristic ring-shaped structures called heat shock granules. Small HSPs contains a-crystallin domain” (ACD) C-terminal domain, N-terminal domain and C-terminal extension (CTE).

Spécificité

The antibody also reacts with a dimer of the HSP 25 (approx. 58 kDa) appearing in preparations stored for prolonged periods prior to electrophoretic analysis. A major proteolytic fragment of 22 kDa may also be detected. The antibody suppresses the inhibition of actin elongation induced by the 25 kDa IAP. The antibody displays a punctate or fine fibrillary pattern of labeling throughout the cytoplasm in heat-shocked cells and co-localization with mitochondria. Reactivity has been observed with HSP 25 of various organs.

Immunogène

partially purified inhibitor of actin polymerization (IAP) protein from turkey gizzard smooth muscle.

Application

Monoclonal Anti-Heat Shock Protein 25 antibody produced in mouse has been used in:

• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• radioimmunoassay (RIA)
• immunocytochemistry
• immunohistofluorescence assay
• immunopurification

Actions biochimiques/physiologiques

Small heat shock proteins (HSPs) play a vital role in stress tolerance. They act as molecular chaperones in vitro. Murine Hsp25 averts aggregation of citrate synthase and α-glucosidase. It also inhibits actin polymerization and the amyloid fibril formation of Aβ1-42. Mutations in human sHsps result in myopathies, neuropathies and cataract. The small HSPs have also been implicated in phenomena of acquired thermotolerance and neoplasia.

Forme physique

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Stockage et stabilité

For continuous use, store at 2-8 °C. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable