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GE17-5132-01

Glutathione Sepharose™ 4 Fast Flow

Cytiva 17-5132-01, pack of 25 mL

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

glutathione and 10-carbon linker arm

Conditionnement

pack of 25 mL

Fabricant/nom de marque

Cytiva 17-5132-01

Matrice

highly cross-linked 4% agarose

Diamètre moyen

90 μm

cleaning

3-12

working range

3-12

Capacité

>10 mg/mL binding capacity

Catégories apparentées

Description générale

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose™ 4 Fast Flow, 25 mL
Glutathione Sepharose™ 4 Fast Flow is an affinity medium for fast, one-step purification of Glutathione S-Transferase (GST) tagged proteins. Provides good flow properties, excellent for scale-up.

Glutathione Sepharose™ 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Sepharose™ 4 Fast Flow provides good flow properties and is excellent for scaling up.

Caractéristiques et avantages

  • Fast, one-step purification of GST-tagged proteins
  • Mild elution conditions preserving protein antigenicity and function
  • For scale-up due to high flow properties
  • Glutathione coupled to Sepharose™ 4 Fast Flow via a 10-carbon spacer arm

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

General Considerations for Purification of GST-tagged Proteins

This page describes general considerations for purification of GST-tagged proteins using Cytiva products.

Purification of GST-tagged Protein

GST-tagged protein yield varies based on protein, host cell, and culture conditions, influencing production.

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.

Protocoles

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

Purification Using GST HiTrap® 1 mL and 5 mL Columns: GSTrap™ 4B, GSTrap™ FF, and GSTrap™ HP

GSTrap™ affinity columns are specially designed 1 ml and 5 ml HiTrap® columns packed with Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, or Glutathione Sepharose 4B media.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

Separation with Cytiva Products (GST Fusion Proteins)

Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.

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