GE11-0034-95
HiTrap® MabSelect SuRe™
Cytiva 11-0034-95, pack of 5 × 5 mL
Synonyme(s) :
prepacked columns, MabSelecT columns
About This Item
Produits recommandés
ligand
alkali-stabilized protein A-derived (E. coli)
Conditionnement
pack of 5 × 5 mL
Fabricant/nom de marque
Cytiva 11-0034-95
Conditions de stockage
(20% Ehtanol)
(20% Ethanol)
Paramètres
flow rate
42 psi
Taille du lit
16 mm × 25 mm
Volume du lit
5 mL
D.I. de la colonne
16 mm
Matrice
highly cross-linked agarose
Taille moy. des particules
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning
2-13.7
working range
3-12
Capacité
≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
Adéquation
suitable for bioprocess medium
Température de stockage
2-8°C
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Catégories apparentées
Description générale
HiTrap® MabSelect™ SuRe™ columns are prepacked with MabSelect™ SuRe, a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allow cleaning-in-place (CIP).
HiTrap® MabSelect™ columns are prepacked with MabSelect™ a bioprocess medium for capuring of Mabs from large sample volumes.
Caractéristiques et avantages
- Withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH.
- Novel ligand design gives enhanced protease resistance resulting in lower ligand leakage.
- Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
- Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- Prepacked HiTrap® columns for process development, screening of purification conditions, and small-scale purification of Mabs.
Stockage et stabilité
Remarque sur l'analyse
Autres remarques
Informations légales
"MabSelect SuRe Ligand Restricted License" and "Cys-rProtein A Ligand Restricted License" are protected by the following patents and equivalent patents and patent applications in other countries: US 6,399,750 and EP 1123389. A free, non-transferable limited license to use this product for internal analytical purposes only accompanies the purchase of the product from a Cytiva company and its licensed distributors. Any other use will require a separate license from a Cytiva company.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
3 - Flammable liquids
Certificats d'analyse (COA)
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Articles
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
Protocoles
This page shows how to separate IgG antibodies by affinity chromatography using MabSelect Sure from Cytiva.
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