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F8666

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O55:B5

FITC conjugate

Synonyme(s) :

LPS

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5 MG
472,00 €
10 MG
571,00 €

About This Item

Numéro CAS:
93572-42-0
Numéro CE :
297-473-0
Numéro MDL:
MFCD00164401
Code UNSPSC :
12352201
Nomenclature NACRES :
NA.25

472,00 €

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Devis pour commande en gros

Source biologique

Escherichia coli (O55:B5)

Niveau de qualité

200

Conjugué

FITC conjugate

Forme

lyophilized powder

Ampleur du marquage

2-10 μg FITC per mg LPS

Couleur

yellow to orange

Solubilité

H2O: 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

This product is a TCA extract of E. coli serotype O55:B5 (strain CDC 1644-70), which was purified by gel filtration and conjugated to FITC (fluorescein isothiocyanate).

Application

Les lipopolysaccharides (LPS) sont des constituants caractéristiques de la paroi cellulaire des bactéries à Gram négatif. Les LPS et leur fraction lipidique (lipide A) stimulent les cellules du système immunitaire inné via le récepteur TLR4 (Toll-Like Receptor 4), un membre de la famille des récepteurs de type Toll, qui reconnaît la signature moléculaire des principaux agents pathogènes (PAMP).
FITC-Lipopolysaccharides (FITC-LPS) may be used to study the movement/transport of LPS across tissue barriers such as the colonic epithelium and cellular binding and internalization of lipopolysaccharides with fluorescence-based assay systems.[1][2] FITC-LPS may be used to evaluate, qualify and quantify lipopolysaccharides (LPS) removal reagents and procedures.[3] FITC-LPS conjugate may be used for the detection of LPA binding proteins and motifs.[4]

Actions biochimiques/physiologiques

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Notes préparatoires

The product is soluble in water (5 mg/ml), yielding a hazy faint yellow solution. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Autres remarques

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

L4524

Lipopolysaccharides from Escherichia coli O55:B5, purified by ion-exchange chromatography, TLR ligand tested

L4005

Lipopolysaccharides from Escherichia coli O55:B5, purified by trichloroacetic acid extraction

L6529

Lipopolysaccharides from Escherichia coli O55:B5, γ-irradiated, BioXtra, suitable for cell culture

L2637

Lipopolysaccharides from Escherichia coli O55:B5, purified by gel-filtration chromatography

L2880

Lipopolysaccharides from Escherichia coli O55:B5, purified by phenol extraction

F8666

Lipopolysaccharides from Escherichia coli O55:B5, FITC conjugate

L5418

Lipopolysaccharides from Escherichia coli O55:B5, Ready Made solution, 1 mg/mL

Pictogrammes

Skull and crossbones
GHS06

Mention d'avertissement

Danger

Mentions de danger

H300

Classification des risques

Acute Tox. 2 Oral

Code de la classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
0000367380
0000130338
0000367380
0000119071
0000093835

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Tatyana Y Postnikova et al.
Pharmaceuticals (Basel, Switzerland), 13(3) (2020-03-22)
Infectious diseases in early postnatal ontogenesis often result in cognitive impairments, particularly learning and memory. The essential foundation of learning and memory is long-term synaptic plasticity, which depends on N-methyl-D-aspartate (NMDA) receptors. In the present study, bacterial infection was modeled
Feifei Wang et al.
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi, 30(3), 635-640 (2013-07-20)
In order to remove the endotoxin from the blood of endotoxemia patients, we prepared a new adsorbent with heparin space arm and polymyxin B (PMB) ligand. The carrier of chloromethyl polystyrene resin was activated and heparin space arm was grafted
Bin Li et al.
International journal of molecular sciences, 15(1), 1143-1161 (2014-01-21)
Innate immunity is the first line of defense in human beings against pathogen infection; monocytes/macrophages are the primary cells of the innate immune system. Recently, macrophages/monocytes have been discovered to participate in LPS clearance, and the clearance efficiency determines the
Elena Topchiy et al.
PloS one, 11(5), e0155030-e0155030 (2016-05-14)
Sepsis is the leading cause of death in critically ill patients. While decreased Proprotein Convertase Subtilisin/Kexin type 9 (PCSK9) function improves clinical outcomes in murine and human sepsis, the mechanisms involved have not been fully elucidated. We tested the hypothesis
Yang Yu et al.
Scientific reports, 6, 20845-20845 (2016-02-10)
Phospholipid transfer protein (PLTP) participates in high density lipoprotein (HDL) metabolism. Increased plasma PLTP activity was observed in lipopolysaccharide (LPS) triggered acute inflammatory diseases. This study aimed to determine the exact role of PLTP in LPS induced inflammation. HDL pool
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Articles

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Questions

  1. What is the molecular weight?

    1 answer

    1. The molecular weight of this product is not determined. However, LPS is heterogeneous and tends to form aggregates of varying sizes that may range from 1-4 million or greater. When treated with SDS and heat the molecular weight range is approximately 50 to 100 kDa. The FITC used to prepare this product has a molecular weight of 389.38 g/mol. There are approximately 3.6 ug FITC per milligram LPS in this preparation.

      Please see the link below to learn more about Lipopolysaccharides:
      https://www.sigmaaldrich.com/technical-documents/protocol/clinical-testing-and-diagnostics-manufacturing/bacteriology/lipopolysaccharides

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