Accéder au contenu
Merck
Toutes les photos(3)

Principaux documents

Voir toute la documentation

F6008

Sigma-Aldrich

Ficin from fig tree latex

lyophilized powder

Synonyme(s) :

Debricin, Ficain, higueroxyl delabarre

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme

Sélectionner une taille de conditionnement

100 UNITS
183,00 €
200 UNITS
332,00 €
1000 UNITS
1 380,00 €

183,00 €

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros
Toutes les photos(3)

Sélectionner une taille de conditionnement

Changer de vue
100 UNITS
183,00 €
200 UNITS
332,00 €
1000 UNITS
1 380,00 €

About This Item

Numéro CAS:
9001-33-6
Numéro de classification (Commission des enzymes):
3.4.22.3 (BRENDA, IUBMB)
Numéro CE :
232-599-1
Numéro MDL:
MFCD00081597
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

183,00 €

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros

Source biologique

fig tree (latex)

Forme

lyophilized powder

Activité spécifique

≥1.0 units/mg protein

Poids mol.

23.8 kDa

Composition

Protein, 80-100% biuret

Température de stockage

−20°C

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Catégories apparentées

Description générale

Extinction Coefficient: E1% = 21.0 (280 nm)
pI: 9.0

Application

Ficin from fig tree latex has been used:
  • in the proteolysis of tendon collagen[1]
  • in the digestion of Chlorophyta Ulva rigida[2]
  • to investigate it effect on protease-activated receptors 2 and 4 activation to induce itch in skin[3]

Actions biochimiques/physiologiques

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)2 fragments from mouse IgG1.
Ficin is effective in disrupting the staphylococcal biofilms making it accessible antibiotics exposure.[4]

Définition de l'unité

One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37°C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).

Forme physique

Lyophilized powder containing L-cysteine and trace EDTA

Notes préparatoires

This enzyme is soluble in water (10 mg/ml), yielding a clear solution.

Inhibiteur

Réf. du produit
Description
Tarif

E3132

E-64, protease inhibitor

Substrat

Réf. du produit
Description
Tarif

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Faites votre choix parmi les versions les plus récentes :

Certificats d'analyse (COA)

Lot/Batch Number
0000416232
0000416232
0000385817
0000335828
0000335827

Vous ne trouvez pas la bonne version ?

Si vous avez besoin d'une version particulière, vous pouvez rechercher un certificat spécifique par le numéro de lot.

Rechercher un(e) COA

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

A Aktypis et al.
Journal of applied microbiology, 84(4), 568-576 (1998-06-20)
ACA-DC 0040 produced an antimicrobial agent, which was named thermophilin T, active against several lactic acid bacteria strains of different species and food spoilage bacteria, such as Clostridium sporogenes C22/10 and Cl. tyrobutyricum NCDO-1754. The crude antimicrobial compound is sensitive
Generation of an artificial skin construct containing a non-degradable fiber mesh: a potential transcutaneous interface
Cahn F and Kyriakides TR
Biomedical Materials (Bristol, England), 3(3), 034110-034110 (2008)
Targeting microbial biofilms using Ficin, a nonspecific plant protease
Baidamshina DR, et al.
Scientific reports, 7, 46068-46068 (2017)
Yusei Takemoto et al.
Journal of cellular physiology, 234(2), 1689-1698 (2018-08-03)
Nuclear factor erythroid 2-related factor 2 (Nrf2) is a master regulator for the induction of antioxidative genes and plays roles in diverse cellular functions. The roles of Nrf2 in muscle regeneration have been investigated, and both important and unimportant roles
Isolation and characterization of angiotensin I-converting enzyme (ACE) inhibitory peptides from Ulva rigida C. Agardh protein hydrolysate
Paiva L, et al.
Journal of functional foods, 26, 65-76 (2016)
Afficher tous les articles scientifiques apparentés

Protocoles

Enzymatic Assay of Ficin (EC 3.4.22.3)

This procedure may be used for all Ficin products.

Questions

  1. What is this used for exactly? In lay terms please.

    1 answer

    1. Ficin is an enzyme that has a number of uses in several markets. As mentioned in the DESCRIPTION section of the product detail page, ficin from fig tree latex has been used:

      in the proteolysis of tendon collagen (tendons are softened in meat/muscles)
      in the digestion of Chlorophyta Ulva rigida (softens this algae which is used in 'sea salad' and animal feed)
      to investigate it's effect on protease-activated receptors 2 and 4 activation to induce itch in skin (studies indicate it can cause itching)
      in disrupting the staphylococcal biofilms, making it accessible antibiotics exposure. (Staph can be very resistant to antibiotics, particularly in 'biofilms'. Ficin digests or breaks down this film so that antibiotics are more effective.)

      It is also used in the food industry, primarily dairy products and is useful in clotting milk to aid in cheese production.
      Ficin has several medical uses, as well. This product is for research use only, however the link below describes some of the medical applications of Ficin:
      https://www.webmd.com/vitamins/ai/ingredientmono-141/ficin

      Helpful?

Reviews

No rating value

Active Filters

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique